Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium
Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy sourc...
Published in: | Applied and Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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American Society for Microbiology
1990
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Online Access: | http://dx.doi.org/10.1128/aem.56.2.315-322.1990 https://journals.asm.org/doi/pdf/10.1128/aem.56.2.315-322.1990 |
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crasmicro:10.1128/aem.56.2.315-322.1990 2023-11-05T03:42:57+01:00 Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium Huber, Harald Stetter, Karl O. 1990 http://dx.doi.org/10.1128/aem.56.2.315-322.1990 https://journals.asm.org/doi/pdf/10.1128/aem.56.2.315-322.1990 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 56, issue 2, page 315-322 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1990 crasmicro https://doi.org/10.1128/aem.56.2.315-322.1990 2023-10-09T15:50:11Z Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that they belong to the genus Thiobacillus. Alternatively, they grow on organic substrates such as yeast extract, peptone, and pyruvate. In contrast to the other leaching bacteria known so far, the new isolates are unable to oxidize ferrous iron. They consist of extreme and moderate acidophiles growing optimally at pH 3 and 4, respectively. The extreme acidophiles showed leaching characteristics similar to those shown by Thiobacillus ferrooxidans , while the moderate acidophiles exhibited a pronounced preference for copper leaching on some chalcopyrite ores. The G+C content of the DNA is between 66 and 69 mol%, depending on the isolate. In DNA-DNA hybridization experiments, the new strains showed homologies among each other of >70%, indicating that they belong to the same species. No significant DNA homology to Thiobacillus reference strains was detectable. Therefore, the new isolates represent a new species of Thiobacillus , which we name Thiobacillus cuprinus . Isolate H�5 is designated as the type strain (DSM 5495). Article in Journal/Newspaper Iceland ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 56 2 315 322 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Huber, Harald Stetter, Karl O. Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that they belong to the genus Thiobacillus. Alternatively, they grow on organic substrates such as yeast extract, peptone, and pyruvate. In contrast to the other leaching bacteria known so far, the new isolates are unable to oxidize ferrous iron. They consist of extreme and moderate acidophiles growing optimally at pH 3 and 4, respectively. The extreme acidophiles showed leaching characteristics similar to those shown by Thiobacillus ferrooxidans , while the moderate acidophiles exhibited a pronounced preference for copper leaching on some chalcopyrite ores. The G+C content of the DNA is between 66 and 69 mol%, depending on the isolate. In DNA-DNA hybridization experiments, the new strains showed homologies among each other of >70%, indicating that they belong to the same species. No significant DNA homology to Thiobacillus reference strains was detectable. Therefore, the new isolates represent a new species of Thiobacillus , which we name Thiobacillus cuprinus . Isolate H�5 is designated as the type strain (DSM 5495). |
format |
Article in Journal/Newspaper |
author |
Huber, Harald Stetter, Karl O. |
author_facet |
Huber, Harald Stetter, Karl O. |
author_sort |
Huber, Harald |
title |
Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium |
title_short |
Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium |
title_full |
Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium |
title_fullStr |
Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium |
title_full_unstemmed |
Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium |
title_sort |
thiobacillus cuprinus sp. nov., a novel facultatively organotrophic metal-mobilizing bacterium |
publisher |
American Society for Microbiology |
publishDate |
1990 |
url |
http://dx.doi.org/10.1128/aem.56.2.315-322.1990 https://journals.asm.org/doi/pdf/10.1128/aem.56.2.315-322.1990 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Applied and Environmental Microbiology volume 56, issue 2, page 315-322 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.56.2.315-322.1990 |
container_title |
Applied and Environmental Microbiology |
container_volume |
56 |
container_issue |
2 |
container_start_page |
315 |
op_container_end_page |
322 |
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1781700582719881216 |