Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture
Aquaculture has brought about increased interest in mass production of marine fish larvae. Problems such as poor egg quality and mass mortality of fish larvae have been prevalent. The intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal re...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.55.6.1435-1446.1989 https://journals.asm.org/doi/pdf/10.1128/aem.55.6.1435-1446.1989 |
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crasmicro:10.1128/aem.55.6.1435-1446.1989 2024-06-23T07:52:56+00:00 Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture Hansen, Geir Høvik Olafsen, Jan A. 1989 http://dx.doi.org/10.1128/aem.55.6.1435-1446.1989 https://journals.asm.org/doi/pdf/10.1128/aem.55.6.1435-1446.1989 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 55, issue 6, page 1435-1446 ISSN 0099-2240 1098-5336 journal-article 1989 crasmicro https://doi.org/10.1128/aem.55.6.1435-1446.1989 2024-06-03T08:10:42Z Aquaculture has brought about increased interest in mass production of marine fish larvae. Problems such as poor egg quality and mass mortality of fish larvae have been prevalent. The intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. Little information about the adherent microflora on fish eggs is available, and the present study was undertaken to describe the microbial ecology during egg development and hatching of two fish species of potential commercial importance in marine aquaculture. Attachment and development of the bacterial flora on cod ( Gadus morhua L.) eggs from fertilization until hatching was studied by scanning electron microscopy. The adherent microflora on cod ( G. morhua L.) and halibut ( Hippoglossus hippoglossus ) eggs during incubation was characterized and grouped by cluster analysis. Marked bacterial growth could be demonstrated 2 h after fertilization, and at hatching eggs were heavily overgrown. Members of the genera Pseudomonas, Alteromonas, Aeromonas , and Flavobacterium were found to dominate on the surface of both cod and halibut eggs. The filamentous bacterium Leucothrix mucor was found on eggs from both species. While growth of L. mucor on halibut eggs was sparse, cod eggs with a hairy appearance due to overgrowth by this bacterium close to hatching were frequently observed. Vibrio fischeri could be detected on cod eggs only, and pathogenic vibrios were not detected. Members of the genera Moraxella and Alcaligenes were found only on halibut eggs. Caulobacter and Seliberia spp. were observed attached to eggs dissected from cod ovaries under sterile conditions, indicating the presence of these bacteria in ovaries before spawning. Adherent strains did not demonstrate antibiotic resistance above a normal level. Attempts to regulate the egg microflora by incubation of ... Article in Journal/Newspaper Gadus morhua ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 55 6 1435 1446 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
Aquaculture has brought about increased interest in mass production of marine fish larvae. Problems such as poor egg quality and mass mortality of fish larvae have been prevalent. The intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. Little information about the adherent microflora on fish eggs is available, and the present study was undertaken to describe the microbial ecology during egg development and hatching of two fish species of potential commercial importance in marine aquaculture. Attachment and development of the bacterial flora on cod ( Gadus morhua L.) eggs from fertilization until hatching was studied by scanning electron microscopy. The adherent microflora on cod ( G. morhua L.) and halibut ( Hippoglossus hippoglossus ) eggs during incubation was characterized and grouped by cluster analysis. Marked bacterial growth could be demonstrated 2 h after fertilization, and at hatching eggs were heavily overgrown. Members of the genera Pseudomonas, Alteromonas, Aeromonas , and Flavobacterium were found to dominate on the surface of both cod and halibut eggs. The filamentous bacterium Leucothrix mucor was found on eggs from both species. While growth of L. mucor on halibut eggs was sparse, cod eggs with a hairy appearance due to overgrowth by this bacterium close to hatching were frequently observed. Vibrio fischeri could be detected on cod eggs only, and pathogenic vibrios were not detected. Members of the genera Moraxella and Alcaligenes were found only on halibut eggs. Caulobacter and Seliberia spp. were observed attached to eggs dissected from cod ovaries under sterile conditions, indicating the presence of these bacteria in ovaries before spawning. Adherent strains did not demonstrate antibiotic resistance above a normal level. Attempts to regulate the egg microflora by incubation of ... |
format |
Article in Journal/Newspaper |
author |
Hansen, Geir Høvik Olafsen, Jan A. |
spellingShingle |
Hansen, Geir Høvik Olafsen, Jan A. Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture |
author_facet |
Hansen, Geir Høvik Olafsen, Jan A. |
author_sort |
Hansen, Geir Høvik |
title |
Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture |
title_short |
Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture |
title_full |
Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture |
title_fullStr |
Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture |
title_full_unstemmed |
Bacterial Colonization of Cod ( Gadus morhua L.) and Halibut ( Hippoglossus hippoglossus ) Eggs in Marine Aquaculture |
title_sort |
bacterial colonization of cod ( gadus morhua l.) and halibut ( hippoglossus hippoglossus ) eggs in marine aquaculture |
publisher |
American Society for Microbiology |
publishDate |
1989 |
url |
http://dx.doi.org/10.1128/aem.55.6.1435-1446.1989 https://journals.asm.org/doi/pdf/10.1128/aem.55.6.1435-1446.1989 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Applied and Environmental Microbiology volume 55, issue 6, page 1435-1446 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.55.6.1435-1446.1989 |
container_title |
Applied and Environmental Microbiology |
container_volume |
55 |
container_issue |
6 |
container_start_page |
1435 |
op_container_end_page |
1446 |
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1802644382422138880 |