Virulence studies based on plasmid profiles of the fish pathogen Vibrio salmonicida

• Published in: Applied and Environmental Microbiology
• Main Authors: Wiik, R, Andersen, K, Daae, F L, Hoff, K A
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 1989
• Subjects: Ecology; Applied Microbiology and Biotechnology; Food Science; Biotechnology; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1128/aem.55.4.819-825.1989; https://journals.asm.org/doi/pdf/10.1128/aem.55.4.819-825.1989

Strains of Vibrio salmonicida isolated from Atlantic salmon (Salmo salar) and rainbow trout (Salmo gairdneri) suffering from cold-water vibriosis could be divided on the basis of plasmid profiles into four different categories. Of 32 strains, 19% harbored three plasmids of 24, 3.4, and 26 megadaltons (MDa), 69% harbored the 24- and 3.4-MDa plasmids but not the 2.6-MDA plasmid, and 9% harbored only the 24-MDA plasmid. The fourth category, which consisted of only one strain, harbored a plasmid of 10 MDa. In spite of different plasmid patterns, the strains of V. salmonicida were very similar with respect to biochemical reactions. The one-third of the V. salmonicida strains which were serotyped were of the same type. The 50% lethal doses, which were determined by intraperitoneal injection, ranged from 4 x 106 to 1 x 108 CFU per fish.