Elevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas)

• Published in: Applied and Environmental Microbiology
• Main Authors: DePaola, A, Kaysner, C A, McPhearson, R M
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 1987
• Subjects: Crassostrea gigas
• Online Access: http://dx.doi.org/10.1128/aem.53.5.1181-1182.1987; https://journals.asm.org/doi/pdf/10.1128/aem.53.5.1181-1182.1987

Of 222 Vibrio cholerae isolates from diverse clinical and environmental sources, 219 produced visible growth in alkaline peptone broth when incubated overnight at 42 degrees C. In field trials conducted to compare enrichment at incubation temperatures of 42 and 35 degrees C, significantly higher rates of isolation (P less than 0.05) and recovery (P less than 0.01) of V. cholerae from oysters were observed at 42 degrees C.