Preservation of ATP in Hypersaline Environments
High concentrations of particulate ATP were found in the anoxic brines of the Orca Basin and East Flower Garden, Gulf of Mexico. Other measurements indicative of growth and respiration suggested that the microbial community in the brines was inactive, but somehow the ATP associated with the cells pe...
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American Society for Microbiology
1987
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Online Access: | http://dx.doi.org/10.1128/aem.53.12.2749-2753.1987 https://journals.asm.org/doi/pdf/10.1128/aem.53.12.2749-2753.1987 |
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crasmicro:10.1128/aem.53.12.2749-2753.1987 2023-11-05T03:44:31+01:00 Preservation of ATP in Hypersaline Environments Tuovila, Bruce J. Dobbs, Fred C. LaRock, Paul A. Siegel, B. Z. 1987 http://dx.doi.org/10.1128/aem.53.12.2749-2753.1987 https://journals.asm.org/doi/pdf/10.1128/aem.53.12.2749-2753.1987 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 53, issue 12, page 2749-2753 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1987 crasmicro https://doi.org/10.1128/aem.53.12.2749-2753.1987 2023-10-09T16:02:03Z High concentrations of particulate ATP were found in the anoxic brines of the Orca Basin and East Flower Garden, Gulf of Mexico. Other measurements indicative of growth and respiration suggested that the microbial community in the brines was inactive, but somehow the ATP associated with the cells persisted. Conceivably, when cells growing just above the interface sank into the brine, the increased osmotic stress could elicit an osmoregulatory response resulting in increased ATP. It was also possible that hydrolytic enzymes were inactivated, resulting in the preservation of ATP. Experiments in which a culture of marine bacteria was suspended in menstrua of different salinities comparable to those found across the Orca Basin interface revealed that as salinity increased, ATP increased three- to sixfold. Within 24 h the ATP fell to its initial level and remained at that concentration for 3 days, at which time the experiment was terminated. In contrast, the control suspensions, at a salinity of 28% (grams per liter) had 1/10th of the initial ATP concentration when the experiment was ended. Cells were also exposed to killing UV irradiation, enabling us to demonstrate with absolute certainty that cellular ATP could be preserved. At the end of the experiment, the viable component of the population was reduced by orders of magnitude by UV irradiation, but the ATP levels of the cells suspended in brine did not decrease. In certain environments it appears that the conventional analytical tools of the microbial ecologist must be interpreted with caution. Article in Journal/Newspaper Orca ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 53 12 2749 2753 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Tuovila, Bruce J. Dobbs, Fred C. LaRock, Paul A. Siegel, B. Z. Preservation of ATP in Hypersaline Environments |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
High concentrations of particulate ATP were found in the anoxic brines of the Orca Basin and East Flower Garden, Gulf of Mexico. Other measurements indicative of growth and respiration suggested that the microbial community in the brines was inactive, but somehow the ATP associated with the cells persisted. Conceivably, when cells growing just above the interface sank into the brine, the increased osmotic stress could elicit an osmoregulatory response resulting in increased ATP. It was also possible that hydrolytic enzymes were inactivated, resulting in the preservation of ATP. Experiments in which a culture of marine bacteria was suspended in menstrua of different salinities comparable to those found across the Orca Basin interface revealed that as salinity increased, ATP increased three- to sixfold. Within 24 h the ATP fell to its initial level and remained at that concentration for 3 days, at which time the experiment was terminated. In contrast, the control suspensions, at a salinity of 28% (grams per liter) had 1/10th of the initial ATP concentration when the experiment was ended. Cells were also exposed to killing UV irradiation, enabling us to demonstrate with absolute certainty that cellular ATP could be preserved. At the end of the experiment, the viable component of the population was reduced by orders of magnitude by UV irradiation, but the ATP levels of the cells suspended in brine did not decrease. In certain environments it appears that the conventional analytical tools of the microbial ecologist must be interpreted with caution. |
format |
Article in Journal/Newspaper |
author |
Tuovila, Bruce J. Dobbs, Fred C. LaRock, Paul A. Siegel, B. Z. |
author_facet |
Tuovila, Bruce J. Dobbs, Fred C. LaRock, Paul A. Siegel, B. Z. |
author_sort |
Tuovila, Bruce J. |
title |
Preservation of ATP in Hypersaline Environments |
title_short |
Preservation of ATP in Hypersaline Environments |
title_full |
Preservation of ATP in Hypersaline Environments |
title_fullStr |
Preservation of ATP in Hypersaline Environments |
title_full_unstemmed |
Preservation of ATP in Hypersaline Environments |
title_sort |
preservation of atp in hypersaline environments |
publisher |
American Society for Microbiology |
publishDate |
1987 |
url |
http://dx.doi.org/10.1128/aem.53.12.2749-2753.1987 https://journals.asm.org/doi/pdf/10.1128/aem.53.12.2749-2753.1987 |
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Orca |
genre_facet |
Orca |
op_source |
Applied and Environmental Microbiology volume 53, issue 12, page 2749-2753 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.53.12.2749-2753.1987 |
container_title |
Applied and Environmental Microbiology |
container_volume |
53 |
container_issue |
12 |
container_start_page |
2749 |
op_container_end_page |
2753 |
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1781704604260499456 |