Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake
We sought to determine factors relating to the survival of heterotrophic microorganisms from the high-dissolved-oxygen (HDO) waters of Lake Hoare, Antarctica. This lake contains perpetual HDO about three times that of normal saturation (40 to 50 mg liter −1 ). Five isolates, one yeast and four bacte...
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American Society for Microbiology
1986
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Online Access: | http://dx.doi.org/10.1128/aem.52.6.1236-1241.1986 https://journals.asm.org/doi/pdf/10.1128/aem.52.6.1236-1241.1986 |
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crasmicro:10.1128/aem.52.6.1236-1241.1986 2023-05-15T14:11:11+02:00 Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake Mikell, Alfred T. Parker, B. C. Gregory, E. M. 1986 http://dx.doi.org/10.1128/aem.52.6.1236-1241.1986 https://journals.asm.org/doi/pdf/10.1128/aem.52.6.1236-1241.1986 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 52, issue 6, page 1236-1241 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1986 crasmicro https://doi.org/10.1128/aem.52.6.1236-1241.1986 2022-10-30T09:13:45Z We sought to determine factors relating to the survival of heterotrophic microorganisms from the high-dissolved-oxygen (HDO) waters of Lake Hoare, Antarctica. This lake contains perpetual HDO about three times that of normal saturation (40 to 50 mg liter −1 ). Five isolates, one yeast and four bacteria, were selected from Lake Hoare waters by growth with the membrane filter technique with oxygen added to yield dissolved concentrations 14 times that in situ, 175 mg liter −1 . One bacterial isolate was obtained from the microbial mat beneath the HDO waters. This organism was isolated at normal atmospheric oxygen saturation. The bacteria were gram-negative rods, motile, oxidase positive, catalase positive, and superoxide dismutase positive; they contained carotenoids. The planktonic isolates grew in media containing 10 mg of Trypticase soy (BBL Microbiology Systems)-peptone (2:1) liter −1 but not at 10 g liter −1 . Under low-nutrient levels simulating Lake Hoare waters (10 mg liter −1 ), two of the planktonic isolates tested were not inhibited by HDO. Growth inhibition by HDO increased as nutrient concentration was increased. A carotenoid-negative mutant of one isolate demonstrated a decreased growth rate, maximal cell density, and increased cell lysis in the death phase under HDO compared with the parent strain. The specific activity of superoxide dismutase was increased by HDO in four of the five bacterial isolates. The superoxide dismutase was of the manganese type on the basis of inhibition and electrophoretic studies. The bacterial isolates from Lake Hoare possess several adaptations which may aid their survival in the HDO waters, as well as protection due to the oligotrophic nature of the lake. Article in Journal/Newspaper Antarc* Antarctic Antarctica ASM Journals (American Society for Microbiology - via Crossref) Antarctic Hoare ENVELOPE(162.850,162.850,-77.633,-77.633) Lake Hoare ENVELOPE(162.850,162.850,-77.633,-77.633) Applied and Environmental Microbiology 52 6 1236 1241 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Mikell, Alfred T. Parker, B. C. Gregory, E. M. Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
We sought to determine factors relating to the survival of heterotrophic microorganisms from the high-dissolved-oxygen (HDO) waters of Lake Hoare, Antarctica. This lake contains perpetual HDO about three times that of normal saturation (40 to 50 mg liter −1 ). Five isolates, one yeast and four bacteria, were selected from Lake Hoare waters by growth with the membrane filter technique with oxygen added to yield dissolved concentrations 14 times that in situ, 175 mg liter −1 . One bacterial isolate was obtained from the microbial mat beneath the HDO waters. This organism was isolated at normal atmospheric oxygen saturation. The bacteria were gram-negative rods, motile, oxidase positive, catalase positive, and superoxide dismutase positive; they contained carotenoids. The planktonic isolates grew in media containing 10 mg of Trypticase soy (BBL Microbiology Systems)-peptone (2:1) liter −1 but not at 10 g liter −1 . Under low-nutrient levels simulating Lake Hoare waters (10 mg liter −1 ), two of the planktonic isolates tested were not inhibited by HDO. Growth inhibition by HDO increased as nutrient concentration was increased. A carotenoid-negative mutant of one isolate demonstrated a decreased growth rate, maximal cell density, and increased cell lysis in the death phase under HDO compared with the parent strain. The specific activity of superoxide dismutase was increased by HDO in four of the five bacterial isolates. The superoxide dismutase was of the manganese type on the basis of inhibition and electrophoretic studies. The bacterial isolates from Lake Hoare possess several adaptations which may aid their survival in the HDO waters, as well as protection due to the oligotrophic nature of the lake. |
format |
Article in Journal/Newspaper |
author |
Mikell, Alfred T. Parker, B. C. Gregory, E. M. |
author_facet |
Mikell, Alfred T. Parker, B. C. Gregory, E. M. |
author_sort |
Mikell, Alfred T. |
title |
Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake |
title_short |
Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake |
title_full |
Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake |
title_fullStr |
Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake |
title_full_unstemmed |
Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake |
title_sort |
factors affecting high-oxygen survival of heterotrophic microorganisms from an antarctic lake |
publisher |
American Society for Microbiology |
publishDate |
1986 |
url |
http://dx.doi.org/10.1128/aem.52.6.1236-1241.1986 https://journals.asm.org/doi/pdf/10.1128/aem.52.6.1236-1241.1986 |
long_lat |
ENVELOPE(162.850,162.850,-77.633,-77.633) ENVELOPE(162.850,162.850,-77.633,-77.633) |
geographic |
Antarctic Hoare Lake Hoare |
geographic_facet |
Antarctic Hoare Lake Hoare |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Applied and Environmental Microbiology volume 52, issue 6, page 1236-1241 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.52.6.1236-1241.1986 |
container_title |
Applied and Environmental Microbiology |
container_volume |
52 |
container_issue |
6 |
container_start_page |
1236 |
op_container_end_page |
1241 |
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1766283302755368960 |