Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic
Root-associated bacteria capable of reducing acetylene to ethylene (biological nitrogen fixation) were isolated from various native plants grown in the Canadian High Arctic. All the strains belonged to the genus Pseudomonas but varied in several physiological characteristics. The rates of acetylene...
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American Society for Microbiology
1986
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Online Access: | http://dx.doi.org/10.1128/aem.51.2.251-255.1986 https://journals.asm.org/doi/pdf/10.1128/aem.51.2.251-255.1986 |
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crasmicro:10.1128/aem.51.2.251-255.1986 2024-06-23T07:49:59+00:00 Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic Lifshitz, Ran Kloepper, Joseph W. Scher, Fran M. Tipping, Elizabeth M. Laliberté, Monique 1986 http://dx.doi.org/10.1128/aem.51.2.251-255.1986 https://journals.asm.org/doi/pdf/10.1128/aem.51.2.251-255.1986 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 51, issue 2, page 251-255 ISSN 0099-2240 1098-5336 journal-article 1986 crasmicro https://doi.org/10.1128/aem.51.2.251-255.1986 2024-06-03T08:10:30Z Root-associated bacteria capable of reducing acetylene to ethylene (biological nitrogen fixation) were isolated from various native plants grown in the Canadian High Arctic. All the strains belonged to the genus Pseudomonas but varied in several physiological characteristics. The rates of acetylene reduction at 14 or 20°C were higher than at 25 or 9°C. Six strains reduced acetylene at 4°C. All the strains exhibited chemotaxis to l -asparagine in semisolid agar at 4 to 25°C. Eleven strains colonized roots of canola ( Brassica campestris cv. Tobin) in field soil at population densities of log 4.3 to log 5.1 CFU/g of fresh root at 14°C and log 4.0 to log 5.2 CFU/g of fresh root at 25°C. Some of these nitrogen-fixing pseudomonad strains demonstrated a competitive advantage for root colonization over other rhizosphere bacteria at low temperatures. The combined capabilities of nitrogen fixation and root colonization by diazotrophic pseudomonads may be useful for the development of a biofertilizer inoculant for temperate and cold regions. Article in Journal/Newspaper Arctic ASM Journals (American Society for Microbiology) Arctic Applied and Environmental Microbiology 51 2 251 255 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
Root-associated bacteria capable of reducing acetylene to ethylene (biological nitrogen fixation) were isolated from various native plants grown in the Canadian High Arctic. All the strains belonged to the genus Pseudomonas but varied in several physiological characteristics. The rates of acetylene reduction at 14 or 20°C were higher than at 25 or 9°C. Six strains reduced acetylene at 4°C. All the strains exhibited chemotaxis to l -asparagine in semisolid agar at 4 to 25°C. Eleven strains colonized roots of canola ( Brassica campestris cv. Tobin) in field soil at population densities of log 4.3 to log 5.1 CFU/g of fresh root at 14°C and log 4.0 to log 5.2 CFU/g of fresh root at 25°C. Some of these nitrogen-fixing pseudomonad strains demonstrated a competitive advantage for root colonization over other rhizosphere bacteria at low temperatures. The combined capabilities of nitrogen fixation and root colonization by diazotrophic pseudomonads may be useful for the development of a biofertilizer inoculant for temperate and cold regions. |
format |
Article in Journal/Newspaper |
author |
Lifshitz, Ran Kloepper, Joseph W. Scher, Fran M. Tipping, Elizabeth M. Laliberté, Monique |
spellingShingle |
Lifshitz, Ran Kloepper, Joseph W. Scher, Fran M. Tipping, Elizabeth M. Laliberté, Monique Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic |
author_facet |
Lifshitz, Ran Kloepper, Joseph W. Scher, Fran M. Tipping, Elizabeth M. Laliberté, Monique |
author_sort |
Lifshitz, Ran |
title |
Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic |
title_short |
Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic |
title_full |
Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic |
title_fullStr |
Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic |
title_full_unstemmed |
Nitrogen-Fixing Pseudomonads Isolated from Roots of Plants Grown in the Canadian High Arctic |
title_sort |
nitrogen-fixing pseudomonads isolated from roots of plants grown in the canadian high arctic |
publisher |
American Society for Microbiology |
publishDate |
1986 |
url |
http://dx.doi.org/10.1128/aem.51.2.251-255.1986 https://journals.asm.org/doi/pdf/10.1128/aem.51.2.251-255.1986 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Applied and Environmental Microbiology volume 51, issue 2, page 251-255 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.51.2.251-255.1986 |
container_title |
Applied and Environmental Microbiology |
container_volume |
51 |
container_issue |
2 |
container_start_page |
251 |
op_container_end_page |
255 |
_version_ |
1802640727947083776 |