Bacterial Plasmids in Antarctic Natural Microbial Assemblages
Samples of psychrophilic and psychrotrophic bacteria were collected from sea ice, seawater, sediments, and benthic or ice-associated animals in McMurdo Sound, Antarctica. A total of 155 strains were isolated and tested for the presence of plasmids by DNA agarose gel electrophoresis. Thirty-one perce...
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American Society for Microbiology
1984
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Online Access: | http://dx.doi.org/10.1128/aem.48.3.515-518.1984 https://journals.asm.org/doi/pdf/10.1128/aem.48.3.515-518.1984 |
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crasmicro:10.1128/aem.48.3.515-518.1984 2024-06-23T07:46:14+00:00 Bacterial Plasmids in Antarctic Natural Microbial Assemblages Kobori, Hiromi Sullivan, Cornelius W. Shizuya, Hiroaki 1984 http://dx.doi.org/10.1128/aem.48.3.515-518.1984 https://journals.asm.org/doi/pdf/10.1128/aem.48.3.515-518.1984 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 48, issue 3, page 515-518 ISSN 0099-2240 1098-5336 journal-article 1984 crasmicro https://doi.org/10.1128/aem.48.3.515-518.1984 2024-06-03T08:10:51Z Samples of psychrophilic and psychrotrophic bacteria were collected from sea ice, seawater, sediments, and benthic or ice-associated animals in McMurdo Sound, Antarctica. A total of 155 strains were isolated and tested for the presence of plasmids by DNA agarose gel electrophoresis. Thirty-one percent of the isolates carried at least one kind of plasmid. Bacterial isolates taken from sediments showed the highest plasmid incidence (42%), and isolates from seawater showed the lowest plasmid incidence (20%). Plasmids were significantly more frequent in the strains which had been first isolated from low-nutrient media (46%) than in the strains which had been isolated from high-nutrient media (25%). Multiple forms of plasmids were observed in two-thirds of the plasmid-carrying strains. A majority of the plasmids detected were estimated to have a mass of 10 megadaltons or less. Among 48 plasmid-carrying strains, 7 showed antibiotic resistance. It is concluded that bacterial plasmids are ubiquitous in natural microbial assemblages of the pristine marine ecosystem of Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Sound Sea ice ASM Journals (American Society for Microbiology) Antarctic McMurdo Sound Applied and Environmental Microbiology 48 3 515 518 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
Samples of psychrophilic and psychrotrophic bacteria were collected from sea ice, seawater, sediments, and benthic or ice-associated animals in McMurdo Sound, Antarctica. A total of 155 strains were isolated and tested for the presence of plasmids by DNA agarose gel electrophoresis. Thirty-one percent of the isolates carried at least one kind of plasmid. Bacterial isolates taken from sediments showed the highest plasmid incidence (42%), and isolates from seawater showed the lowest plasmid incidence (20%). Plasmids were significantly more frequent in the strains which had been first isolated from low-nutrient media (46%) than in the strains which had been isolated from high-nutrient media (25%). Multiple forms of plasmids were observed in two-thirds of the plasmid-carrying strains. A majority of the plasmids detected were estimated to have a mass of 10 megadaltons or less. Among 48 plasmid-carrying strains, 7 showed antibiotic resistance. It is concluded that bacterial plasmids are ubiquitous in natural microbial assemblages of the pristine marine ecosystem of Antarctica. |
format |
Article in Journal/Newspaper |
author |
Kobori, Hiromi Sullivan, Cornelius W. Shizuya, Hiroaki |
spellingShingle |
Kobori, Hiromi Sullivan, Cornelius W. Shizuya, Hiroaki Bacterial Plasmids in Antarctic Natural Microbial Assemblages |
author_facet |
Kobori, Hiromi Sullivan, Cornelius W. Shizuya, Hiroaki |
author_sort |
Kobori, Hiromi |
title |
Bacterial Plasmids in Antarctic Natural Microbial Assemblages |
title_short |
Bacterial Plasmids in Antarctic Natural Microbial Assemblages |
title_full |
Bacterial Plasmids in Antarctic Natural Microbial Assemblages |
title_fullStr |
Bacterial Plasmids in Antarctic Natural Microbial Assemblages |
title_full_unstemmed |
Bacterial Plasmids in Antarctic Natural Microbial Assemblages |
title_sort |
bacterial plasmids in antarctic natural microbial assemblages |
publisher |
American Society for Microbiology |
publishDate |
1984 |
url |
http://dx.doi.org/10.1128/aem.48.3.515-518.1984 https://journals.asm.org/doi/pdf/10.1128/aem.48.3.515-518.1984 |
geographic |
Antarctic McMurdo Sound |
geographic_facet |
Antarctic McMurdo Sound |
genre |
Antarc* Antarctic Antarctica McMurdo Sound Sea ice |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Sound Sea ice |
op_source |
Applied and Environmental Microbiology volume 48, issue 3, page 515-518 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.48.3.515-518.1984 |
container_title |
Applied and Environmental Microbiology |
container_volume |
48 |
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3 |
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515 |
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518 |
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1802644711562805248 |