In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit
Bituminous hydrocarbons extracted from the Athabasca oil sands of north-eastern Alberta were adsorbed onto filter supports and placed at sites in the Athabasca River and its tributaries where these rivers come in contact with the oil sands formation. Colonization of the hydrocarbon surfaces at summe...
Published in: | Applied and Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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American Society for Microbiology
1981
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Online Access: | http://dx.doi.org/10.1128/aem.41.3.791-800.1981 https://journals.asm.org/doi/pdf/10.1128/aem.41.3.791-800.1981 |
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crasmicro:10.1128/aem.41.3.791-800.1981 2023-11-05T03:40:20+01:00 In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit Wyndham, R. C. Costerton, J. W. 1981 http://dx.doi.org/10.1128/aem.41.3.791-800.1981 https://journals.asm.org/doi/pdf/10.1128/aem.41.3.791-800.1981 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 41, issue 3, page 791-800 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1981 crasmicro https://doi.org/10.1128/aem.41.3.791-800.1981 2023-10-09T16:06:19Z Bituminous hydrocarbons extracted from the Athabasca oil sands of north-eastern Alberta were adsorbed onto filter supports and placed at sites in the Athabasca River and its tributaries where these rivers come in contact with the oil sands formation. Colonization of the hydrocarbon surfaces at summer and winter ambient temperatures was examined by scanning and transmission electron microscopy as well as by epifluorescence microscopy of acridine orange-stained cross sections. Ruthenium red and alkaline bismuth stains visualized an association of bacteria with the hydrocarbon surface which was mediated by bacterial polysaccharides. Bacteria apparently lacking a glycocalyx were also found closely associated with the surface of the hydrophobic substrate and in channels within the substrate. A solvent precipitation and column chromatographic fractionation of the bitumen was followed by cross-tests for growth on the fractions by various isolated sediment microorganisms, as determined by epifluorescence count. All fractions except the asphaltenes supported the growth of at least two of the isolates, although fractionation of degraded bitumen revealed that the saturate, aromatic, and first polar fractions were preferentially degraded. Article in Journal/Newspaper Athabasca River ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 41 3 791 800 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Wyndham, R. C. Costerton, J. W. In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Bituminous hydrocarbons extracted from the Athabasca oil sands of north-eastern Alberta were adsorbed onto filter supports and placed at sites in the Athabasca River and its tributaries where these rivers come in contact with the oil sands formation. Colonization of the hydrocarbon surfaces at summer and winter ambient temperatures was examined by scanning and transmission electron microscopy as well as by epifluorescence microscopy of acridine orange-stained cross sections. Ruthenium red and alkaline bismuth stains visualized an association of bacteria with the hydrocarbon surface which was mediated by bacterial polysaccharides. Bacteria apparently lacking a glycocalyx were also found closely associated with the surface of the hydrophobic substrate and in channels within the substrate. A solvent precipitation and column chromatographic fractionation of the bitumen was followed by cross-tests for growth on the fractions by various isolated sediment microorganisms, as determined by epifluorescence count. All fractions except the asphaltenes supported the growth of at least two of the isolates, although fractionation of degraded bitumen revealed that the saturate, aromatic, and first polar fractions were preferentially degraded. |
format |
Article in Journal/Newspaper |
author |
Wyndham, R. C. Costerton, J. W. |
author_facet |
Wyndham, R. C. Costerton, J. W. |
author_sort |
Wyndham, R. C. |
title |
In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit |
title_short |
In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit |
title_full |
In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit |
title_fullStr |
In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit |
title_full_unstemmed |
In Vitro Microbial Degradation of Bituminous Hydrocarbons and In Situ Colonization of Bitumen Surfaces Within the Athabasca Oil Sands Deposit |
title_sort |
in vitro microbial degradation of bituminous hydrocarbons and in situ colonization of bitumen surfaces within the athabasca oil sands deposit |
publisher |
American Society for Microbiology |
publishDate |
1981 |
url |
http://dx.doi.org/10.1128/aem.41.3.791-800.1981 https://journals.asm.org/doi/pdf/10.1128/aem.41.3.791-800.1981 |
genre |
Athabasca River |
genre_facet |
Athabasca River |
op_source |
Applied and Environmental Microbiology volume 41, issue 3, page 791-800 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.41.3.791-800.1981 |
container_title |
Applied and Environmental Microbiology |
container_volume |
41 |
container_issue |
3 |
container_start_page |
791 |
op_container_end_page |
800 |
_version_ |
1781696362910318592 |