Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities
Alkaline phosphatase (APase) activity was detected in aquatic microbial assemblages from the subtropics to Antarctica. The occurrence of APase in environmental nucleotide extracts was shown to significantly affect the measured concentrations of cellular nucleotides (adenosine triphosphate, adenosine...
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Language: | English |
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American Society for Microbiology
1980
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Online Access: | http://dx.doi.org/10.1128/aem.40.3.549-561.1980 https://journals.asm.org/doi/pdf/10.1128/aem.40.3.549-561.1980 |
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crasmicro:10.1128/aem.40.3.549-561.1980 2023-11-05T03:36:10+01:00 Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities Karl, D. M. Craven, D. B. 1980 http://dx.doi.org/10.1128/aem.40.3.549-561.1980 https://journals.asm.org/doi/pdf/10.1128/aem.40.3.549-561.1980 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 40, issue 3, page 549-561 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1980 crasmicro https://doi.org/10.1128/aem.40.3.549-561.1980 2023-10-09T16:02:42Z Alkaline phosphatase (APase) activity was detected in aquatic microbial assemblages from the subtropics to Antarctica. The occurrence of APase in environmental nucleotide extracts was shown to significantly affect the measured concentrations of cellular nucleotides (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, guanosine triphosphate, uridine triphosphate, and cytidine triphosphate), adenylate energy charge, and guanosine triphosphate/adenosine triphosphate ratios, when conventional methods of nucleotide extraction were employed. Under the reaction conditions specified in this report, the initial rate of hydrolysis of adenosine triphosphate was directly proportional to the activity of APase in the sample extracts and consequently can be used as a sensitive measure of APase activity. A method was devised for obtaining reliable nucleotide measurements in naturally occurring microbial populations containing elevated levels of APase activity. The metabolic significance of APase activity in microbial cells is discussed, and it is concluded that the occurrence and regulation of APase in nature is dependent upon microscale inorganic phosphate limitation of the autochthonous microbial communities. Article in Journal/Newspaper Antarc* Antarctica ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 40 3 549 561 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Karl, D. M. Craven, D. B. Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Alkaline phosphatase (APase) activity was detected in aquatic microbial assemblages from the subtropics to Antarctica. The occurrence of APase in environmental nucleotide extracts was shown to significantly affect the measured concentrations of cellular nucleotides (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, guanosine triphosphate, uridine triphosphate, and cytidine triphosphate), adenylate energy charge, and guanosine triphosphate/adenosine triphosphate ratios, when conventional methods of nucleotide extraction were employed. Under the reaction conditions specified in this report, the initial rate of hydrolysis of adenosine triphosphate was directly proportional to the activity of APase in the sample extracts and consequently can be used as a sensitive measure of APase activity. A method was devised for obtaining reliable nucleotide measurements in naturally occurring microbial populations containing elevated levels of APase activity. The metabolic significance of APase activity in microbial cells is discussed, and it is concluded that the occurrence and regulation of APase in nature is dependent upon microscale inorganic phosphate limitation of the autochthonous microbial communities. |
format |
Article in Journal/Newspaper |
author |
Karl, D. M. Craven, D. B. |
author_facet |
Karl, D. M. Craven, D. B. |
author_sort |
Karl, D. M. |
title |
Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities |
title_short |
Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities |
title_full |
Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities |
title_fullStr |
Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities |
title_full_unstemmed |
Effects of Alkaline Phosphatase Activity on Nucleotide Measurements in Aquatic Microbial Communities |
title_sort |
effects of alkaline phosphatase activity on nucleotide measurements in aquatic microbial communities |
publisher |
American Society for Microbiology |
publishDate |
1980 |
url |
http://dx.doi.org/10.1128/aem.40.3.549-561.1980 https://journals.asm.org/doi/pdf/10.1128/aem.40.3.549-561.1980 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Applied and Environmental Microbiology volume 40, issue 3, page 549-561 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.40.3.549-561.1980 |
container_title |
Applied and Environmental Microbiology |
container_volume |
40 |
container_issue |
3 |
container_start_page |
549 |
op_container_end_page |
561 |
_version_ |
1781690873790070784 |