Growth of Fish Cell Lines on Microcarriers
Microcarrier beads were evaluated as substrates for the propagation of five anchorage-dependent fish cell lines. Growth of rainbow trout gonad (RTG-2) and Atlantic salmon cells was limited on microcarriers maintained in suspension. However, stationary microcarriers were suitable substrates for the g...
Published in: | Applied and Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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American Society for Microbiology
1980
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Online Access: | http://dx.doi.org/10.1128/aem.39.2.394-397.1980 https://journals.asm.org/doi/pdf/10.1128/aem.39.2.394-397.1980 |
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crasmicro:10.1128/aem.39.2.394-397.1980 2024-09-15T17:56:23+00:00 Growth of Fish Cell Lines on Microcarriers Nicholson, Bruce L. 1980 http://dx.doi.org/10.1128/aem.39.2.394-397.1980 https://journals.asm.org/doi/pdf/10.1128/aem.39.2.394-397.1980 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 39, issue 2, page 394-397 ISSN 0099-2240 1098-5336 journal-article 1980 crasmicro https://doi.org/10.1128/aem.39.2.394-397.1980 2024-07-22T04:08:35Z Microcarrier beads were evaluated as substrates for the propagation of five anchorage-dependent fish cell lines. Growth of rainbow trout gonad (RTG-2) and Atlantic salmon cells was limited on microcarriers maintained in suspension. However, stationary microcarriers were suitable substrates for the growth of RTG-2, AS, Chinook salmon embryo (CHSE-214), and fathead minnow cells. Cell yields ranged from 2 × 10 6 to 2.9 × 10 6 cells per ml, representing 7- to 10-fold increases over the initial cell concentrations. The yield of new RTG-2 cells per unit volume of growth medium was 2.8 times greater in microcarrier cultures than in standard monolayer cultures. Northern pike cells failed to grow on microcarriers. Yields of infectious pancreatic necrosis virus propagated in microcarrier cultures of RTG-2 cells were more than twice the yields in standard monolayer cultures. The greater economy of microcarrier cultures in terms of growth vessel and medium requirements holds great promise for the large-scale production of anchorage-dependent fish cell cultures and fish viruses. Article in Journal/Newspaper Atlantic salmon ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 39 2 394 397 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
Microcarrier beads were evaluated as substrates for the propagation of five anchorage-dependent fish cell lines. Growth of rainbow trout gonad (RTG-2) and Atlantic salmon cells was limited on microcarriers maintained in suspension. However, stationary microcarriers were suitable substrates for the growth of RTG-2, AS, Chinook salmon embryo (CHSE-214), and fathead minnow cells. Cell yields ranged from 2 × 10 6 to 2.9 × 10 6 cells per ml, representing 7- to 10-fold increases over the initial cell concentrations. The yield of new RTG-2 cells per unit volume of growth medium was 2.8 times greater in microcarrier cultures than in standard monolayer cultures. Northern pike cells failed to grow on microcarriers. Yields of infectious pancreatic necrosis virus propagated in microcarrier cultures of RTG-2 cells were more than twice the yields in standard monolayer cultures. The greater economy of microcarrier cultures in terms of growth vessel and medium requirements holds great promise for the large-scale production of anchorage-dependent fish cell cultures and fish viruses. |
format |
Article in Journal/Newspaper |
author |
Nicholson, Bruce L. |
spellingShingle |
Nicholson, Bruce L. Growth of Fish Cell Lines on Microcarriers |
author_facet |
Nicholson, Bruce L. |
author_sort |
Nicholson, Bruce L. |
title |
Growth of Fish Cell Lines on Microcarriers |
title_short |
Growth of Fish Cell Lines on Microcarriers |
title_full |
Growth of Fish Cell Lines on Microcarriers |
title_fullStr |
Growth of Fish Cell Lines on Microcarriers |
title_full_unstemmed |
Growth of Fish Cell Lines on Microcarriers |
title_sort |
growth of fish cell lines on microcarriers |
publisher |
American Society for Microbiology |
publishDate |
1980 |
url |
http://dx.doi.org/10.1128/aem.39.2.394-397.1980 https://journals.asm.org/doi/pdf/10.1128/aem.39.2.394-397.1980 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Applied and Environmental Microbiology volume 39, issue 2, page 394-397 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.39.2.394-397.1980 |
container_title |
Applied and Environmental Microbiology |
container_volume |
39 |
container_issue |
2 |
container_start_page |
394 |
op_container_end_page |
397 |
_version_ |
1810432593168957440 |