Modified procedure for the recovery of naturally accumulated poliovirus from oysters
Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitatio...
Published in: | Applied and Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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American Society for Microbiology
1979
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Online Access: | http://dx.doi.org/10.1128/aem.38.4.594-598.1979 https://journals.asm.org/doi/pdf/10.1128/aem.38.4.594-598.1979 |
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crasmicro:10.1128/aem.38.4.594-598.1979 2023-06-11T04:11:05+02:00 Modified procedure for the recovery of naturally accumulated poliovirus from oysters Vaughn, J M Landry, E F Vicale, T J Dahl, M C 1979 http://dx.doi.org/10.1128/aem.38.4.594-598.1979 https://journals.asm.org/doi/pdf/10.1128/aem.38.4.594-598.1979 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 38, issue 4, page 594-598 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1979 crasmicro https://doi.org/10.1128/aem.38.4.594-598.1979 2023-04-20T12:06:20Z Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters. Article in Journal/Newspaper Crassostrea gigas ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 38 4 594 598 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Vaughn, J M Landry, E F Vicale, T J Dahl, M C Modified procedure for the recovery of naturally accumulated poliovirus from oysters |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters. |
format |
Article in Journal/Newspaper |
author |
Vaughn, J M Landry, E F Vicale, T J Dahl, M C |
author_facet |
Vaughn, J M Landry, E F Vicale, T J Dahl, M C |
author_sort |
Vaughn, J M |
title |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters |
title_short |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters |
title_full |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters |
title_fullStr |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters |
title_full_unstemmed |
Modified procedure for the recovery of naturally accumulated poliovirus from oysters |
title_sort |
modified procedure for the recovery of naturally accumulated poliovirus from oysters |
publisher |
American Society for Microbiology |
publishDate |
1979 |
url |
http://dx.doi.org/10.1128/aem.38.4.594-598.1979 https://journals.asm.org/doi/pdf/10.1128/aem.38.4.594-598.1979 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Applied and Environmental Microbiology volume 38, issue 4, page 594-598 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.38.4.594-598.1979 |
container_title |
Applied and Environmental Microbiology |
container_volume |
38 |
container_issue |
4 |
container_start_page |
594 |
op_container_end_page |
598 |
_version_ |
1768385940712062976 |