Modified procedure for the recovery of naturally accumulated poliovirus from oysters

Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitatio...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Vaughn, J M, Landry, E F, Vicale, T J, Dahl, M C
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 1979
Subjects:
Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Crassostrea gigas
Online Access:http://dx.doi.org/10.1128/aem.38.4.594-598.1979
https://journals.asm.org/doi/pdf/10.1128/aem.38.4.594-598.1979
Description
Summary:Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters.

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