Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab
Two variations of the multiple-tube fermentation technique were used to enumerate fecal coliforms in commercially processed, frozen crab meat. These were the EC confirmation test and a more rapid method that requires medium A-1. The method with medium A-1 was more specific than the EC confirmation t...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
1979
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Online Access: | http://dx.doi.org/10.1128/aem.37.5.836-840.1979 https://journals.asm.org/doi/pdf/10.1128/aem.37.5.836-840.1979 |
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crasmicro:10.1128/aem.37.5.836-840.1979 2023-10-09T21:55:55+02:00 Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab Powell, J C Moore, A R Gow, J A 1979 http://dx.doi.org/10.1128/aem.37.5.836-840.1979 https://journals.asm.org/doi/pdf/10.1128/aem.37.5.836-840.1979 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 37, issue 5, page 836-840 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 1979 crasmicro https://doi.org/10.1128/aem.37.5.836-840.1979 2023-09-15T05:47:06Z Two variations of the multiple-tube fermentation technique were used to enumerate fecal coliforms in commercially processed, frozen crab meat. These were the EC confirmation test and a more rapid method that requires medium A-1. The method with medium A-1 was more specific than the EC confirmation test for detecting Escherichia coli type 1. E. coli was isolated from 84% of the positive medium A-1 tubes, whereas it was isolated from only 64% of the positive tubes of EC broth. When samples of crab meat were inoculated with known amounts of E. coli, better estimates of the known numbers were obtained by the medium A-1 method. Several species of nonfecal coliforms were isolated from cultures in EC broth. These belonged to the genera Klebsiella, Citrobacter, Enterobacter, and Serratia. Apparently these strains were naturally adapted to growth at an elevated temperature because the majority were able to grow at 44.5 degrees C when retested in EC broth. Fewer species of nonfecal coliforms were isolated from medium A-1. Those that were isolated belonged to the genera Citrobacter and Enterobacter. Article in Journal/Newspaper Snow crab ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 37 5 836 840 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Powell, J C Moore, A R Gow, J A Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
Two variations of the multiple-tube fermentation technique were used to enumerate fecal coliforms in commercially processed, frozen crab meat. These were the EC confirmation test and a more rapid method that requires medium A-1. The method with medium A-1 was more specific than the EC confirmation test for detecting Escherichia coli type 1. E. coli was isolated from 84% of the positive medium A-1 tubes, whereas it was isolated from only 64% of the positive tubes of EC broth. When samples of crab meat were inoculated with known amounts of E. coli, better estimates of the known numbers were obtained by the medium A-1 method. Several species of nonfecal coliforms were isolated from cultures in EC broth. These belonged to the genera Klebsiella, Citrobacter, Enterobacter, and Serratia. Apparently these strains were naturally adapted to growth at an elevated temperature because the majority were able to grow at 44.5 degrees C when retested in EC broth. Fewer species of nonfecal coliforms were isolated from medium A-1. Those that were isolated belonged to the genera Citrobacter and Enterobacter. |
format |
Article in Journal/Newspaper |
author |
Powell, J C Moore, A R Gow, J A |
author_facet |
Powell, J C Moore, A R Gow, J A |
author_sort |
Powell, J C |
title |
Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab |
title_short |
Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab |
title_full |
Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab |
title_fullStr |
Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab |
title_full_unstemmed |
Comparison of EC broth and medium A-1 for the recovery of Escherichia coli from frozen shucked snow crab |
title_sort |
comparison of ec broth and medium a-1 for the recovery of escherichia coli from frozen shucked snow crab |
publisher |
American Society for Microbiology |
publishDate |
1979 |
url |
http://dx.doi.org/10.1128/aem.37.5.836-840.1979 https://journals.asm.org/doi/pdf/10.1128/aem.37.5.836-840.1979 |
genre |
Snow crab |
genre_facet |
Snow crab |
op_source |
Applied and Environmental Microbiology volume 37, issue 5, page 836-840 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.37.5.836-840.1979 |
container_title |
Applied and Environmental Microbiology |
container_volume |
37 |
container_issue |
5 |
container_start_page |
836 |
op_container_end_page |
840 |
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1779320157142777856 |