Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation
Ant-300, a psychrophilic marine vibrio isolated from the surface water of the Antarctic convergence, was starved for periods of more than 1 year. During the first week of starvation, cell numbers increased from 100 to 800% of the initial number of cells. Fifty percent of the starved cells remained v...
Published in: | Applied and Environmental Microbiology |
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Language: | English |
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American Society for Microbiology
1977
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Online Access: | http://dx.doi.org/10.1128/aem.33.3.635-641.1977 https://journals.asm.org/doi/pdf/10.1128/aem.33.3.635-641.1977 |
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crasmicro:10.1128/aem.33.3.635-641.1977 2024-06-23T07:47:07+00:00 Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation Novitsky, James A. Morita, Richard Y. 1977 http://dx.doi.org/10.1128/aem.33.3.635-641.1977 https://journals.asm.org/doi/pdf/10.1128/aem.33.3.635-641.1977 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 33, issue 3, page 635-641 ISSN 0099-2240 1098-5336 journal-article 1977 crasmicro https://doi.org/10.1128/aem.33.3.635-641.1977 2024-06-10T04:06:58Z Ant-300, a psychrophilic marine vibrio isolated from the surface water of the Antarctic convergence, was starved for periods of more than 1 year. During the first week of starvation, cell numbers increased from 100 to 800% of the initial number of cells. Fifty percent of the starved cells remained viable for 6 to 7 weeks while a portion of the population remained viable for more than 1 year. During the first 2 days of starvation, the endogenous respiration of the cells decreased over 80%. After 7 days, respiration had been reduced to 0.0071% total carbon respired per hour and remained constant thereafter. After 6 weeks of starvation, 46% of the cellular deoxyribonucleic acid had been degraded. Observation of the cellular deoxyribonucleic acid with Feulgen staining before starvation showed the average number of nuclear bodies per cell varied from 1.44 to 4.02 depending on the age of the culture. A linear relationship was found between the number of nuclear bodies per cell and the increase in cell numbers upon starvation. Our data suggest that Ant-300 is capable of surviving long periods of time with little or no nutrients and is therefore well adapted for the sparse nutrient conditions of the colder portions of the open ocean. Article in Journal/Newspaper Antarc* Antarctic ASM Journals (American Society for Microbiology) Antarctic The Antarctic Applied and Environmental Microbiology 33 3 635 641 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
Ant-300, a psychrophilic marine vibrio isolated from the surface water of the Antarctic convergence, was starved for periods of more than 1 year. During the first week of starvation, cell numbers increased from 100 to 800% of the initial number of cells. Fifty percent of the starved cells remained viable for 6 to 7 weeks while a portion of the population remained viable for more than 1 year. During the first 2 days of starvation, the endogenous respiration of the cells decreased over 80%. After 7 days, respiration had been reduced to 0.0071% total carbon respired per hour and remained constant thereafter. After 6 weeks of starvation, 46% of the cellular deoxyribonucleic acid had been degraded. Observation of the cellular deoxyribonucleic acid with Feulgen staining before starvation showed the average number of nuclear bodies per cell varied from 1.44 to 4.02 depending on the age of the culture. A linear relationship was found between the number of nuclear bodies per cell and the increase in cell numbers upon starvation. Our data suggest that Ant-300 is capable of surviving long periods of time with little or no nutrients and is therefore well adapted for the sparse nutrient conditions of the colder portions of the open ocean. |
format |
Article in Journal/Newspaper |
author |
Novitsky, James A. Morita, Richard Y. |
spellingShingle |
Novitsky, James A. Morita, Richard Y. Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation |
author_facet |
Novitsky, James A. Morita, Richard Y. |
author_sort |
Novitsky, James A. |
title |
Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation |
title_short |
Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation |
title_full |
Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation |
title_fullStr |
Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation |
title_full_unstemmed |
Survival of a Psychrophilic Marine Vibrio Under Long-Term Nutrient Starvation |
title_sort |
survival of a psychrophilic marine vibrio under long-term nutrient starvation |
publisher |
American Society for Microbiology |
publishDate |
1977 |
url |
http://dx.doi.org/10.1128/aem.33.3.635-641.1977 https://journals.asm.org/doi/pdf/10.1128/aem.33.3.635-641.1977 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Applied and Environmental Microbiology volume 33, issue 3, page 635-641 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.33.3.635-641.1977 |
container_title |
Applied and Environmental Microbiology |
container_volume |
33 |
container_issue |
3 |
container_start_page |
635 |
op_container_end_page |
641 |
_version_ |
1802651078499500032 |