Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada
ABSTRACT The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the...
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crasmicro:10.1128/aem.05999-11 2024-09-15T18:11:03+00:00 Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. 2013 http://dx.doi.org/10.1128/aem.05999-11 https://journals.asm.org/doi/pdf/10.1128/AEM.05999-11 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 79, issue 2, page 646-654 ISSN 0099-2240 1098-5336 journal-article 2013 crasmicro https://doi.org/10.1128/aem.05999-11 2024-08-19T04:06:17Z ABSTRACT The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson Strait, Hudson Bay, and Ungava Bay regions, respectively. Spores were detected in seawater or coastal rock surfaces from 17.6% of butchering sites, almost all of which were located in southern Ungava Bay. Concentrations of C. botulinum type E along the Ungava Bay coast were significantly higher than on the coasts of Hudson Strait and Hudson Bay, with the highest concentrations (270 to 1,800/kg of sample) found near butchering sites located along the mouths of large rivers. The Koksoak River contained high levels of C. botulinum type E, with the highest median concentration (270/kg) found in sediments of the marine portion of the river. C. botulinum type E was found in the intestinal contents (4.4%) and skins (1.4%) of seals. A high genetic biodiversity of C. botulinum type E isolates was observed among the 21 butchering sites and their surroundings along the Nunavik coastline, with 83% of isolates (44/53) yielding distinct pulsed-field gel electrophoresis genotypes. Multiple sources of C. botulinum type E may be involved in the contamination of seal meat during butchering in this region, but the risk of contamination appears to be much higher from environmental sources along the shoreline of southern Ungava Bay and the sediments of the Koksoak River. Article in Journal/Newspaper Hudson Bay Hudson Strait inuit Ungava Bay Nunavik ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 79 2 646 654 |
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English |
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ABSTRACT The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson Strait, Hudson Bay, and Ungava Bay regions, respectively. Spores were detected in seawater or coastal rock surfaces from 17.6% of butchering sites, almost all of which were located in southern Ungava Bay. Concentrations of C. botulinum type E along the Ungava Bay coast were significantly higher than on the coasts of Hudson Strait and Hudson Bay, with the highest concentrations (270 to 1,800/kg of sample) found near butchering sites located along the mouths of large rivers. The Koksoak River contained high levels of C. botulinum type E, with the highest median concentration (270/kg) found in sediments of the marine portion of the river. C. botulinum type E was found in the intestinal contents (4.4%) and skins (1.4%) of seals. A high genetic biodiversity of C. botulinum type E isolates was observed among the 21 butchering sites and their surroundings along the Nunavik coastline, with 83% of isolates (44/53) yielding distinct pulsed-field gel electrophoresis genotypes. Multiple sources of C. botulinum type E may be involved in the contamination of seal meat during butchering in this region, but the risk of contamination appears to be much higher from environmental sources along the shoreline of southern Ungava Bay and the sediments of the Koksoak River. |
format |
Article in Journal/Newspaper |
author |
Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. |
spellingShingle |
Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
author_facet |
Leclair, Daniel Farber, Jeffrey M. Doidge, Bill Blanchfield, Burke Suppa, Sandy Pagotto, Franco Austin, John W. |
author_sort |
Leclair, Daniel |
title |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_short |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_full |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_fullStr |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_full_unstemmed |
Distribution of Clostridium botulinum Type E Strains in Nunavik, Northern Quebec, Canada |
title_sort |
distribution of clostridium botulinum type e strains in nunavik, northern quebec, canada |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://dx.doi.org/10.1128/aem.05999-11 https://journals.asm.org/doi/pdf/10.1128/AEM.05999-11 |
genre |
Hudson Bay Hudson Strait inuit Ungava Bay Nunavik |
genre_facet |
Hudson Bay Hudson Strait inuit Ungava Bay Nunavik |
op_source |
Applied and Environmental Microbiology volume 79, issue 2, page 646-654 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.05999-11 |
container_title |
Applied and Environmental Microbiology |
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79 |
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2 |
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646 |
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654 |
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1810448646334840832 |