Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish
ABSTRACT The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oys...
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Online Access: | http://dx.doi.org/10.1128/aem.04020-14 https://journals.asm.org/doi/pdf/10.1128/AEM.04020-14 |
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crasmicro:10.1128/aem.04020-14 2024-05-19T07:46:51+00:00 Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish Cruz, C. D. Hedderley, D. Fletcher, G. C. Elkins, C. A. 2015 http://dx.doi.org/10.1128/aem.04020-14 https://journals.asm.org/doi/pdf/10.1128/AEM.04020-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 81, issue 7, page 2320-2327 ISSN 0099-2240 1098-5336 journal-article 2015 crasmicro https://doi.org/10.1128/aem.04020-14 2024-05-02T06:49:22Z ABSTRACT The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels and the prevalence of V. parahaemolyticus tdh and trh strains. Pacific (235) and dredge (21) oyster samples and mussel samples (55) were obtained from commercial shellfish-growing areas between December 2009 and June 2012. Total V. parahaemolyticus numbers and the presence of pathogenic genes tdh and trh were determined using the FDA most-probable-number (MPN) method and confirmed using PCR analysis. In samples from the North Island of NZ, V. parahaemolyticus was detected in 81% of Pacific oysters and 34% of mussel samples, while the numbers of V. parahaemolyticus tdh and trh strains were low, with just 3/215 Pacific oyster samples carrying the tdh gene. V. parahaemolyticus organisms carrying tdh and trh were not detected in South Island samples, and V. parahaemolyticus was detected in just 1/21 dredge oyster and 2/16 mussel samples. Numbers of V. parahaemolyticus organisms increased when seawater temperatures were high, the season when most commercial shellfish-growing areas are not harvested. The numbers of V. parahaemolyticus organisms in samples exceeded 1,000 MPN/g only when the seawater temperatures exceeded 19°C, so this environmental parameter could be used as a trigger warning of potential hazard. There is some evidence that the total V. parahaemolyticus numbers increased compared with those reported from a previous 1981 to 1984 study, but the analytical methods differed significantly. Article in Journal/Newspaper Pacific oyster ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 81 7 2320 2327 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels and the prevalence of V. parahaemolyticus tdh and trh strains. Pacific (235) and dredge (21) oyster samples and mussel samples (55) were obtained from commercial shellfish-growing areas between December 2009 and June 2012. Total V. parahaemolyticus numbers and the presence of pathogenic genes tdh and trh were determined using the FDA most-probable-number (MPN) method and confirmed using PCR analysis. In samples from the North Island of NZ, V. parahaemolyticus was detected in 81% of Pacific oysters and 34% of mussel samples, while the numbers of V. parahaemolyticus tdh and trh strains were low, with just 3/215 Pacific oyster samples carrying the tdh gene. V. parahaemolyticus organisms carrying tdh and trh were not detected in South Island samples, and V. parahaemolyticus was detected in just 1/21 dredge oyster and 2/16 mussel samples. Numbers of V. parahaemolyticus organisms increased when seawater temperatures were high, the season when most commercial shellfish-growing areas are not harvested. The numbers of V. parahaemolyticus organisms in samples exceeded 1,000 MPN/g only when the seawater temperatures exceeded 19°C, so this environmental parameter could be used as a trigger warning of potential hazard. There is some evidence that the total V. parahaemolyticus numbers increased compared with those reported from a previous 1981 to 1984 study, but the analytical methods differed significantly. |
author2 |
Elkins, C. A. |
format |
Article in Journal/Newspaper |
author |
Cruz, C. D. Hedderley, D. Fletcher, G. C. |
spellingShingle |
Cruz, C. D. Hedderley, D. Fletcher, G. C. Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish |
author_facet |
Cruz, C. D. Hedderley, D. Fletcher, G. C. |
author_sort |
Cruz, C. D. |
title |
Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish |
title_short |
Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish |
title_full |
Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish |
title_fullStr |
Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish |
title_full_unstemmed |
Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish |
title_sort |
long-term study of vibrio parahaemolyticus prevalence and distribution in new zealand shellfish |
publisher |
American Society for Microbiology |
publishDate |
2015 |
url |
http://dx.doi.org/10.1128/aem.04020-14 https://journals.asm.org/doi/pdf/10.1128/AEM.04020-14 |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Applied and Environmental Microbiology volume 81, issue 7, page 2320-2327 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.04020-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
81 |
container_issue |
7 |
container_start_page |
2320 |
op_container_end_page |
2327 |
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1799487104926875648 |