Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
ABSTRACT Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis , two predominant zoonotic helminths ci...
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Online Access: | http://dx.doi.org/10.1128/aem.03467-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03467-15 |
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crasmicro:10.1128/aem.03467-15 2024-05-12T08:02:11+00:00 Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence Dozois, C. M. 2016 http://dx.doi.org/10.1128/aem.03467-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03467-15 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 82, issue 10, page 2950-2958 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2016 crasmicro https://doi.org/10.1128/aem.03467-15 2024-04-18T06:52:38Z ABSTRACT Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis , two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes , Canis lupus familiaris , Canis lupus , Felis silvestris catus , Meles meles , Martes foina , and Martes martes . With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed ( E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens. Article in Journal/Newspaper Canis lupus ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 82 10 2950 2958 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis , two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes , Canis lupus familiaris , Canis lupus , Felis silvestris catus , Meles meles , Martes foina , and Martes martes . With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed ( E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens. |
author2 |
Dozois, C. M. |
format |
Article in Journal/Newspaper |
author |
Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence |
author_facet |
Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence |
author_sort |
Knapp, Jenny |
title |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_short |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_full |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_fullStr |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_full_unstemmed |
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis |
title_sort |
development of a real-time pcr for a sensitive one-step coprodiagnosis allowing both the identification of carnivore feces and the detection of toxocara spp. and echinococcus multilocularis |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
http://dx.doi.org/10.1128/aem.03467-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03467-15 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Applied and Environmental Microbiology volume 82, issue 10, page 2950-2958 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.03467-15 |
container_title |
Applied and Environmental Microbiology |
container_volume |
82 |
container_issue |
10 |
container_start_page |
2950 |
op_container_end_page |
2958 |
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1798844271484207104 |