Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis

ABSTRACT Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis , two predominant zoonotic helminths ci...

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Published in:Applied and Environmental Microbiology
Main Authors: Knapp, Jenny, Umhang, Gérald, Poulle, Marie-Lazarine, Millon, Laurence
Other Authors: Dozois, C. M.
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2016
Subjects:
Online Access:http://dx.doi.org/10.1128/aem.03467-15
https://journals.asm.org/doi/pdf/10.1128/AEM.03467-15
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spelling crasmicro:10.1128/aem.03467-15 2024-05-12T08:02:11+00:00 Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis Knapp, Jenny Umhang, Gérald Poulle, Marie-Lazarine Millon, Laurence Dozois, C. M. 2016 http://dx.doi.org/10.1128/aem.03467-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03467-15 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 82, issue 10, page 2950-2958 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2016 crasmicro https://doi.org/10.1128/aem.03467-15 2024-04-18T06:52:38Z ABSTRACT Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis , two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes , Canis lupus familiaris , Canis lupus , Felis silvestris catus , Meles meles , Martes foina , and Martes martes . With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed ( E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens. Article in Journal/Newspaper Canis lupus ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 82 10 2950 2958
institution Open Polar
collection ASM Journals (American Society for Microbiology)
op_collection_id crasmicro
language English
topic Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
spellingShingle Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Knapp, Jenny
Umhang, Gérald
Poulle, Marie-Lazarine
Millon, Laurence
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
topic_facet Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
description ABSTRACT Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis , two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes , Canis lupus familiaris , Canis lupus , Felis silvestris catus , Meles meles , Martes foina , and Martes martes . With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed ( E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens.
author2 Dozois, C. M.
format Article in Journal/Newspaper
author Knapp, Jenny
Umhang, Gérald
Poulle, Marie-Lazarine
Millon, Laurence
author_facet Knapp, Jenny
Umhang, Gérald
Poulle, Marie-Lazarine
Millon, Laurence
author_sort Knapp, Jenny
title Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
title_short Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
title_full Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
title_fullStr Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
title_full_unstemmed Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis
title_sort development of a real-time pcr for a sensitive one-step coprodiagnosis allowing both the identification of carnivore feces and the detection of toxocara spp. and echinococcus multilocularis
publisher American Society for Microbiology
publishDate 2016
url http://dx.doi.org/10.1128/aem.03467-15
https://journals.asm.org/doi/pdf/10.1128/AEM.03467-15
genre Canis lupus
genre_facet Canis lupus
op_source Applied and Environmental Microbiology
volume 82, issue 10, page 2950-2958
ISSN 0099-2240 1098-5336
op_rights https://journals.asm.org/non-commercial-tdm-license
op_doi https://doi.org/10.1128/aem.03467-15
container_title Applied and Environmental Microbiology
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