Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields
ABSTRACT Microorganisms are responsible for multiple antibiotic resistances that have been associated with resistance/tolerance to heavy metals, with consequences to public health. Many genes conferring these resistances are located on mobile genetic elements, easily exchanged among phylogenetically...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.03240-14 https://journals.asm.org/doi/pdf/10.1128/AEM.03240-14 |
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crasmicro:10.1128/aem.03240-14 2024-06-23T07:55:13+00:00 Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields Farias, Pedro Espírito Santo, Christophe Branco, Rita Francisco, Romeu Santos, Susana Hansen, Lars Sorensen, Soren Morais, Paula V. Liu, S.-J. 2015 http://dx.doi.org/10.1128/aem.03240-14 https://journals.asm.org/doi/pdf/10.1128/AEM.03240-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 81, issue 7, page 2534-2543 ISSN 0099-2240 1098-5336 journal-article 2015 crasmicro https://doi.org/10.1128/aem.03240-14 2024-06-10T04:07:18Z ABSTRACT Microorganisms are responsible for multiple antibiotic resistances that have been associated with resistance/tolerance to heavy metals, with consequences to public health. Many genes conferring these resistances are located on mobile genetic elements, easily exchanged among phylogenetically distant bacteria. The objective of the present work was to isolate arsenic-, antimonite-, and antibiotic-resistant strains and to determine the existence of plasmids harboring antibiotic/arsenic/antimonite resistance traits in phenotypically resistant strains, in a nonanthropogenically impacted environment. The hydrothermal Lucky Strike field in the Azores archipelago (North Atlantic, between 11°N and 38°N), at the Mid-Atlantic Ridge, protected under the OSPAR Convention, was sampled as a metal-rich pristine environment. A total of 35 strains from 8 different species were isolated in the presence of arsenate, arsenite, and antimonite. ACR3 and arsB genes were amplified from the sediment's total DNA, and 4 isolates also carried ACR3 genes. Phenotypic multiple resistances were found in all strains, and 7 strains had recoverable plasmids. Purified plasmids were sequenced by Illumina and assembled by EDENA V3, and contig annotation was performed using the “Rapid Annotation using the Subsystems Technology” server. Determinants of resistance to copper, zinc, cadmium, cobalt, and chromium as well as to the antibiotics β-lactams and fluoroquinolones were found in the 3 sequenced plasmids. Genes coding for heavy metal resistance and antibiotic resistance in the same mobile element were found, suggesting the possibility of horizontal gene transfer and distribution of theses resistances in the bacterial population. Article in Journal/Newspaper North Atlantic ASM Journals (American Society for Microbiology) Mid-Atlantic Ridge Applied and Environmental Microbiology 81 7 2534 2543 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Microorganisms are responsible for multiple antibiotic resistances that have been associated with resistance/tolerance to heavy metals, with consequences to public health. Many genes conferring these resistances are located on mobile genetic elements, easily exchanged among phylogenetically distant bacteria. The objective of the present work was to isolate arsenic-, antimonite-, and antibiotic-resistant strains and to determine the existence of plasmids harboring antibiotic/arsenic/antimonite resistance traits in phenotypically resistant strains, in a nonanthropogenically impacted environment. The hydrothermal Lucky Strike field in the Azores archipelago (North Atlantic, between 11°N and 38°N), at the Mid-Atlantic Ridge, protected under the OSPAR Convention, was sampled as a metal-rich pristine environment. A total of 35 strains from 8 different species were isolated in the presence of arsenate, arsenite, and antimonite. ACR3 and arsB genes were amplified from the sediment's total DNA, and 4 isolates also carried ACR3 genes. Phenotypic multiple resistances were found in all strains, and 7 strains had recoverable plasmids. Purified plasmids were sequenced by Illumina and assembled by EDENA V3, and contig annotation was performed using the “Rapid Annotation using the Subsystems Technology” server. Determinants of resistance to copper, zinc, cadmium, cobalt, and chromium as well as to the antibiotics β-lactams and fluoroquinolones were found in the 3 sequenced plasmids. Genes coding for heavy metal resistance and antibiotic resistance in the same mobile element were found, suggesting the possibility of horizontal gene transfer and distribution of theses resistances in the bacterial population. |
author2 |
Liu, S.-J. |
format |
Article in Journal/Newspaper |
author |
Farias, Pedro Espírito Santo, Christophe Branco, Rita Francisco, Romeu Santos, Susana Hansen, Lars Sorensen, Soren Morais, Paula V. |
spellingShingle |
Farias, Pedro Espírito Santo, Christophe Branco, Rita Francisco, Romeu Santos, Susana Hansen, Lars Sorensen, Soren Morais, Paula V. Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields |
author_facet |
Farias, Pedro Espírito Santo, Christophe Branco, Rita Francisco, Romeu Santos, Susana Hansen, Lars Sorensen, Soren Morais, Paula V. |
author_sort |
Farias, Pedro |
title |
Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields |
title_short |
Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields |
title_full |
Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields |
title_fullStr |
Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields |
title_full_unstemmed |
Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields |
title_sort |
natural hot spots for gain of multiple resistances: arsenic and antibiotic resistances in heterotrophic, aerobic bacteria from marine hydrothermal vent fields |
publisher |
American Society for Microbiology |
publishDate |
2015 |
url |
http://dx.doi.org/10.1128/aem.03240-14 https://journals.asm.org/doi/pdf/10.1128/AEM.03240-14 |
geographic |
Mid-Atlantic Ridge |
geographic_facet |
Mid-Atlantic Ridge |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Applied and Environmental Microbiology volume 81, issue 7, page 2534-2543 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.03240-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
81 |
container_issue |
7 |
container_start_page |
2534 |
op_container_end_page |
2543 |
_version_ |
1802647704728240128 |