Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway)
ABSTRACT The Adventfjorden time series station (IsA) in Isfjorden, West Spitsbergen, Norway, was sampled frequently from December 2011 to December 2012. The community composition of microbial eukaryotes (size, 0.45 to 10 μm) from a depth of 25 m was determined using 454 sequencing of the 18S V4 regi...
Published in: | Applied and Environmental Microbiology |
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Language: | English |
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Online Access: | http://dx.doi.org/10.1128/aem.03208-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03208-15 |
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crasmicro:10.1128/aem.03208-15 2024-06-23T07:44:59+00:00 Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) Marquardt, Miriam Vader, Anna Stübner, Eike I. Reigstad, Marit Gabrielsen, Tove M. Stams, A. J. M. 2016 http://dx.doi.org/10.1128/aem.03208-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03208-15 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 82, issue 6, page 1868-1880 ISSN 0099-2240 1098-5336 journal-article 2016 crasmicro https://doi.org/10.1128/aem.03208-15 2024-06-10T04:07:29Z ABSTRACT The Adventfjorden time series station (IsA) in Isfjorden, West Spitsbergen, Norway, was sampled frequently from December 2011 to December 2012. The community composition of microbial eukaryotes (size, 0.45 to 10 μm) from a depth of 25 m was determined using 454 sequencing of the 18S V4 region amplified from both DNA and RNA. The compositional changes throughout the year were assessed in relation to in situ fjord environmental conditions. Size fractionation analyses of chlorophyll a showed that the photosynthetic biomass was dominated by small cells (<10 μm) most of the year but that larger cells dominated during the spring and summer. The winter and early-spring communities were more diverse than the spring and summer/autumn communities. Dinophyceae were predominant throughout the year. The Arctic Micromonas ecotype was abundant mostly in the early-bloom and fall periods, whereas heterotrophs, such as marine stramenopiles (MASTs), Picozoa, and the parasitoid marine alveolates (MALVs), displayed higher relative abundance in the winter than in other seasons. Our results emphasize the extreme seasonality of Arctic microbial eukaryotic communities driven by the light regime and nutrient availability but point to the necessity of a thorough knowledge of hydrography for full understanding of their succession and variability. Article in Journal/Newspaper Adventfjorden Arctic Isfjord* Isfjorden Spitsbergen ASM Journals (American Society for Microbiology) Adventfjorden ENVELOPE(15.515,15.515,78.258,78.258) Arctic Norway Applied and Environmental Microbiology 82 6 1868 1880 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT The Adventfjorden time series station (IsA) in Isfjorden, West Spitsbergen, Norway, was sampled frequently from December 2011 to December 2012. The community composition of microbial eukaryotes (size, 0.45 to 10 μm) from a depth of 25 m was determined using 454 sequencing of the 18S V4 region amplified from both DNA and RNA. The compositional changes throughout the year were assessed in relation to in situ fjord environmental conditions. Size fractionation analyses of chlorophyll a showed that the photosynthetic biomass was dominated by small cells (<10 μm) most of the year but that larger cells dominated during the spring and summer. The winter and early-spring communities were more diverse than the spring and summer/autumn communities. Dinophyceae were predominant throughout the year. The Arctic Micromonas ecotype was abundant mostly in the early-bloom and fall periods, whereas heterotrophs, such as marine stramenopiles (MASTs), Picozoa, and the parasitoid marine alveolates (MALVs), displayed higher relative abundance in the winter than in other seasons. Our results emphasize the extreme seasonality of Arctic microbial eukaryotic communities driven by the light regime and nutrient availability but point to the necessity of a thorough knowledge of hydrography for full understanding of their succession and variability. |
author2 |
Stams, A. J. M. |
format |
Article in Journal/Newspaper |
author |
Marquardt, Miriam Vader, Anna Stübner, Eike I. Reigstad, Marit Gabrielsen, Tove M. |
spellingShingle |
Marquardt, Miriam Vader, Anna Stübner, Eike I. Reigstad, Marit Gabrielsen, Tove M. Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) |
author_facet |
Marquardt, Miriam Vader, Anna Stübner, Eike I. Reigstad, Marit Gabrielsen, Tove M. |
author_sort |
Marquardt, Miriam |
title |
Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) |
title_short |
Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) |
title_full |
Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) |
title_fullStr |
Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) |
title_full_unstemmed |
Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway) |
title_sort |
strong seasonality of marine microbial eukaryotes in a high-arctic fjord (isfjorden, in west spitsbergen, norway) |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
http://dx.doi.org/10.1128/aem.03208-15 https://journals.asm.org/doi/pdf/10.1128/AEM.03208-15 |
long_lat |
ENVELOPE(15.515,15.515,78.258,78.258) |
geographic |
Adventfjorden Arctic Norway |
geographic_facet |
Adventfjorden Arctic Norway |
genre |
Adventfjorden Arctic Isfjord* Isfjorden Spitsbergen |
genre_facet |
Adventfjorden Arctic Isfjord* Isfjorden Spitsbergen |
op_source |
Applied and Environmental Microbiology volume 82, issue 6, page 1868-1880 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.03208-15 |
container_title |
Applied and Environmental Microbiology |
container_volume |
82 |
container_issue |
6 |
container_start_page |
1868 |
op_container_end_page |
1880 |
_version_ |
1802643008144801792 |