Strain-Dependent Norovirus Bioaccumulation in Oysters
ABSTRACT Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence...
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crasmicro:10.1128/aem.03010-10 2024-09-15T18:03:15+00:00 Strain-Dependent Norovirus Bioaccumulation in Oysters Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Françoise S. 2011 http://dx.doi.org/10.1128/aem.03010-10 https://journals.asm.org/doi/pdf/10.1128/AEM.03010-10 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 77, issue 10, page 3189-3196 ISSN 0099-2240 1098-5336 journal-article 2011 crasmicro https://doi.org/10.1128/aem.03010-10 2024-08-26T04:06:32Z ABSTRACT Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccumulation. This study compares the bioaccumulation efficiencies and tissue distributions in oysters ( Crassostrea gigas ) of three strains from the two principal human norovirus genogroups. Clear differences between strains were observed. The GI.1 strain was the most efficiently concentrated strain. Bioaccumulation specifically occurred in digestive tissues in a dose-dependent manner, and its efficiency paralleled ligand expression, which was highest during the cold months. In comparison, the GII.4 strain was very poorly bioaccumulated and was recovered in almost all tissues without seasonal influence. The GII.3 strain presented an intermediate behavior, without seasonal effect and with less bioaccumulation efficiency than that of the GI.1 strain during the cold months. In addition, the GII.3 strain was transiently concentrated in gills and mantle before being almost specifically accumulated in digestive tissues. Carbohydrate ligand specificities of the strains at least partly explain the strain-dependent bioaccumulation characteristics. In particular, binding to the digestive-tube-specific ligand should contribute to bioaccumulation, whereas we hypothesize that binding to the sialic acid-containing ligand present in all tissues would contribute to retain virus particles in the gills or mantle and lead to rapid destruction. Article in Journal/Newspaper Crassostrea gigas ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 77 10 3189 3196 |
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ASM Journals (American Society for Microbiology) |
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English |
description |
ABSTRACT Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccumulation. This study compares the bioaccumulation efficiencies and tissue distributions in oysters ( Crassostrea gigas ) of three strains from the two principal human norovirus genogroups. Clear differences between strains were observed. The GI.1 strain was the most efficiently concentrated strain. Bioaccumulation specifically occurred in digestive tissues in a dose-dependent manner, and its efficiency paralleled ligand expression, which was highest during the cold months. In comparison, the GII.4 strain was very poorly bioaccumulated and was recovered in almost all tissues without seasonal influence. The GII.3 strain presented an intermediate behavior, without seasonal effect and with less bioaccumulation efficiency than that of the GI.1 strain during the cold months. In addition, the GII.3 strain was transiently concentrated in gills and mantle before being almost specifically accumulated in digestive tissues. Carbohydrate ligand specificities of the strains at least partly explain the strain-dependent bioaccumulation characteristics. In particular, binding to the digestive-tube-specific ligand should contribute to bioaccumulation, whereas we hypothesize that binding to the sialic acid-containing ligand present in all tissues would contribute to retain virus particles in the gills or mantle and lead to rapid destruction. |
format |
Article in Journal/Newspaper |
author |
Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Françoise S. |
spellingShingle |
Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Françoise S. Strain-Dependent Norovirus Bioaccumulation in Oysters |
author_facet |
Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Françoise S. |
author_sort |
Maalouf, Haifa |
title |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_short |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_full |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_fullStr |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_full_unstemmed |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_sort |
strain-dependent norovirus bioaccumulation in oysters |
publisher |
American Society for Microbiology |
publishDate |
2011 |
url |
http://dx.doi.org/10.1128/aem.03010-10 https://journals.asm.org/doi/pdf/10.1128/AEM.03010-10 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Applied and Environmental Microbiology volume 77, issue 10, page 3189-3196 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.03010-10 |
container_title |
Applied and Environmental Microbiology |
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77 |
container_issue |
10 |
container_start_page |
3189 |
op_container_end_page |
3196 |
_version_ |
1810440769379500032 |