Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales)
ABSTRACT Three cohorts of farmed yellowtail kingfish ( Seriola lalandi ) from South Australia were examined for Chlamydia -like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification,...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.02899-12 https://journals.asm.org/doi/pdf/10.1128/AEM.02899-12 |
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crasmicro:10.1128/aem.02899-12 2024-09-30T14:28:26+00:00 Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. 2013 http://dx.doi.org/10.1128/aem.02899-12 https://journals.asm.org/doi/pdf/10.1128/AEM.02899-12 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 79, issue 5, page 1590-1597 ISSN 0099-2240 1098-5336 journal-article 2013 crasmicro https://doi.org/10.1128/aem.02899-12 2024-09-02T04:05:09Z ABSTRACT Three cohorts of farmed yellowtail kingfish ( Seriola lalandi ) from South Australia were examined for Chlamydia -like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales . A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported “ Candidatus Piscichlamydia salmonis” (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydia -like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales . Based on these observations, we propose this bacterium of yellowtail kingfish be known as “ Candidatus Parilichlamydia carangidicola” and that the new family be known as “ Candidatus Parilichlamydiaceae.” Article in Journal/Newspaper Arctic charr Arctic Atlantic salmon ASM Journals (American Society for Microbiology) Arctic Applied and Environmental Microbiology 79 5 1590 1597 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Three cohorts of farmed yellowtail kingfish ( Seriola lalandi ) from South Australia were examined for Chlamydia -like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the order Chlamydiales . A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported “ Candidatus Piscichlamydia salmonis” (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35 Chlamydia and Chlamydia -like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the order Chlamydiales . Based on these observations, we propose this bacterium of yellowtail kingfish be known as “ Candidatus Parilichlamydia carangidicola” and that the new family be known as “ Candidatus Parilichlamydiaceae.” |
format |
Article in Journal/Newspaper |
author |
Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. |
spellingShingle |
Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
author_facet |
Stride, M. C. Polkinghorne, A. Miller, T. L. Groff, J. M. LaPatra, S. E. Nowak, B. F. |
author_sort |
Stride, M. C. |
title |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_short |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_full |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_fullStr |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_full_unstemmed |
Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales) |
title_sort |
molecular characterization of “candidatus parilichlamydia carangidicola,” a novel chlamydia-like epitheliocystis agent in yellowtail kingfish, seriola lalandi (valenciennes), and the proposal of a new family, “candidatus parilichlamydiaceae” fam. nov. (order chlamydiales) |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://dx.doi.org/10.1128/aem.02899-12 https://journals.asm.org/doi/pdf/10.1128/AEM.02899-12 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Atlantic salmon |
genre_facet |
Arctic charr Arctic Atlantic salmon |
op_source |
Applied and Environmental Microbiology volume 79, issue 5, page 1590-1597 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.02899-12 |
container_title |
Applied and Environmental Microbiology |
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79 |
container_issue |
5 |
container_start_page |
1590 |
op_container_end_page |
1597 |
_version_ |
1811634121679568896 |