Identification of a Novel Alkaliphilic Esterase Active at Low Temperatures by Screening a Metagenomic Library from Antarctic Desert Soil

ABSTRACT A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans . The enzyme was active over a...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Heath, Caroline, Hu, Xiao Ping, Cary, S. Craig, Cowan, Donald
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2009
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Online Access:http://dx.doi.org/10.1128/aem.02597-08
https://journals.asm.org/doi/pdf/10.1128/AEM.02597-08
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Summary:ABSTRACT A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans . The enzyme was active over a temperature range of 7 to 54°C and at alkaline pH levels and is a potential candidate for industrial application.