Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate
ABSTRACT Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant...
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American Society for Microbiology
2007
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Online Access: | http://dx.doi.org/10.1128/aem.02243-06 https://journals.asm.org/doi/pdf/10.1128/AEM.02243-06 |
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crasmicro:10.1128/aem.02243-06 2024-06-23T07:57:20+00:00 Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng 2007 http://dx.doi.org/10.1128/aem.02243-06 https://journals.asm.org/doi/pdf/10.1128/AEM.02243-06 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 73, issue 4, page 1349-1354 ISSN 0099-2240 1098-5336 journal-article 2007 crasmicro https://doi.org/10.1128/aem.02243-06 2024-06-03T08:10:43Z ABSTRACT Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (<0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 10 9 CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity. Article in Journal/Newspaper Turbot ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 73 4 1349 1354 |
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ASM Journals (American Society for Microbiology) |
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English |
description |
ABSTRACT Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (<0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 10 9 CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity. |
format |
Article in Journal/Newspaper |
author |
Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng |
spellingShingle |
Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate |
author_facet |
Du, Meng Chen, Jixiang Zhang, Xiaohua Li, Aijuan Li, Yun Wang, Yingeng |
author_sort |
Du, Meng |
title |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate |
title_short |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate |
title_full |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate |
title_fullStr |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate |
title_full_unstemmed |
Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate |
title_sort |
retention of virulence in a viable but nonculturable edwardsiella tarda isolate |
publisher |
American Society for Microbiology |
publishDate |
2007 |
url |
http://dx.doi.org/10.1128/aem.02243-06 https://journals.asm.org/doi/pdf/10.1128/AEM.02243-06 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Applied and Environmental Microbiology volume 73, issue 4, page 1349-1354 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.02243-06 |
container_title |
Applied and Environmental Microbiology |
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73 |
container_issue |
4 |
container_start_page |
1349 |
op_container_end_page |
1354 |
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1802650924612583424 |