Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties
ABSTRACT Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.02207-14 https://journals.asm.org/doi/pdf/10.1128/AEM.02207-14 |
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crasmicro:10.1128/aem.02207-14 2024-09-15T17:40:30+00:00 Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. Nojiri, H. 2014 http://dx.doi.org/10.1128/aem.02207-14 https://journals.asm.org/doi/pdf/10.1128/AEM.02207-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 80, issue 22, page 7061-7070 ISSN 0099-2240 1098-5336 journal-article 2014 crasmicro https://doi.org/10.1128/aem.02207-14 2024-07-22T04:09:15Z ABSTRACT Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting approach compared to their chemical synthesis. To generate Te-containing nanostructures, bacteria enzymatically reduce tellurite to elemental tellurium. In this work, using a classic biochemical approach, we looked for a novel tellurite reductase from the Antarctic bacterium Pseudomonas sp. strain BNF22 and used it to generate tellurium-containing nanostructures. A new tellurite reductase was identified as glutathione reductase, which was subsequently overproduced in Escherichia coli . The characterization of this enzyme showed that it is an NADPH-dependent tellurite reductase, with optimum reducing activity at 30°C and pH 9.0. Finally, the enzyme was able to generate Te-containing nanostructures, about 68 nm in size, which exhibit interesting antibacterial properties against E. coli , with no apparent cytotoxicity against eukaryotic cells. Article in Journal/Newspaper Antarc* Antarctic ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 80 22 7061 7070 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting approach compared to their chemical synthesis. To generate Te-containing nanostructures, bacteria enzymatically reduce tellurite to elemental tellurium. In this work, using a classic biochemical approach, we looked for a novel tellurite reductase from the Antarctic bacterium Pseudomonas sp. strain BNF22 and used it to generate tellurium-containing nanostructures. A new tellurite reductase was identified as glutathione reductase, which was subsequently overproduced in Escherichia coli . The characterization of this enzyme showed that it is an NADPH-dependent tellurite reductase, with optimum reducing activity at 30°C and pH 9.0. Finally, the enzyme was able to generate Te-containing nanostructures, about 68 nm in size, which exhibit interesting antibacterial properties against E. coli , with no apparent cytotoxicity against eukaryotic cells. |
author2 |
Nojiri, H. |
format |
Article in Journal/Newspaper |
author |
Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. |
spellingShingle |
Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
author_facet |
Pugin, Benoit Cornejo, Fabián A. Muñoz-Díaz, Pablo Muñoz-Villagrán, Claudia M. Vargas-Pérez, Joaquín I. Arenas, Felipe A. Vásquez, Claudio C. |
author_sort |
Pugin, Benoit |
title |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_short |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_full |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_fullStr |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_full_unstemmed |
Glutathione Reductase-Mediated Synthesis of Tellurium-Containing Nanostructures Exhibiting Antibacterial Properties |
title_sort |
glutathione reductase-mediated synthesis of tellurium-containing nanostructures exhibiting antibacterial properties |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://dx.doi.org/10.1128/aem.02207-14 https://journals.asm.org/doi/pdf/10.1128/AEM.02207-14 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Applied and Environmental Microbiology volume 80, issue 22, page 7061-7070 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.02207-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
22 |
container_start_page |
7061 |
op_container_end_page |
7070 |
_version_ |
1810486549942370304 |