The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth
ABSTRACT Psychrobacter arcticus strain 273-4, which grows at temperatures as low as −10°C, is the first cold-adapted bacterium from a terrestrial environment whose genome was sequenced. Analysis of the 2.65-Mb genome suggested that some of the strategies employed by P. arcticus 273-4 for survival un...
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Online Access: | http://dx.doi.org/10.1128/aem.02101-09 https://journals.asm.org/doi/pdf/10.1128/AEM.02101-09 |
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crasmicro:10.1128/aem.02101-09 2024-09-15T18:29:56+00:00 The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth Ayala-del-Río, Héctor L. Chain, Patrick S. Grzymski, Joseph J. Ponder, Monica A. Ivanova, Natalia Bergholz, Peter W. Di Bartolo, Genevive Hauser, Loren Land, Miriam Bakermans, Corien Rodrigues, Debora Klappenbach, Joel Zarka, Dan Larimer, Frank Richardson, Paul Murray, Alison Thomashow, Michael Tiedje, James M. 2010 http://dx.doi.org/10.1128/aem.02101-09 https://journals.asm.org/doi/pdf/10.1128/AEM.02101-09 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 76, issue 7, page 2304-2312 ISSN 0099-2240 1098-5336 journal-article 2010 crasmicro https://doi.org/10.1128/aem.02101-09 2024-08-26T04:06:17Z ABSTRACT Psychrobacter arcticus strain 273-4, which grows at temperatures as low as −10°C, is the first cold-adapted bacterium from a terrestrial environment whose genome was sequenced. Analysis of the 2.65-Mb genome suggested that some of the strategies employed by P. arcticus 273-4 for survival under cold and stress conditions are changes in membrane composition, synthesis of cold shock proteins, and the use of acetate as an energy source. Comparative genome analysis indicated that in a significant portion of the P. arcticus proteome there is reduced use of the acidic amino acids and proline and arginine, which is consistent with increased protein flexibility at low temperatures. Differential amino acid usage occurred in all gene categories, but it was more common in gene categories essential for cell growth and reproduction, suggesting that P. arcticus evolved to grow at low temperatures. Amino acid adaptations and the gene content likely evolved in response to the long-term freezing temperatures (−10°C to −12°C) of the Kolyma (Siberia) permafrost soil from which this strain was isolated. Intracellular water likely does not freeze at these in situ temperatures, which allows P. arcticus to live at subzero temperatures. Article in Journal/Newspaper permafrost Siberia ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 76 7 2304 2312 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Psychrobacter arcticus strain 273-4, which grows at temperatures as low as −10°C, is the first cold-adapted bacterium from a terrestrial environment whose genome was sequenced. Analysis of the 2.65-Mb genome suggested that some of the strategies employed by P. arcticus 273-4 for survival under cold and stress conditions are changes in membrane composition, synthesis of cold shock proteins, and the use of acetate as an energy source. Comparative genome analysis indicated that in a significant portion of the P. arcticus proteome there is reduced use of the acidic amino acids and proline and arginine, which is consistent with increased protein flexibility at low temperatures. Differential amino acid usage occurred in all gene categories, but it was more common in gene categories essential for cell growth and reproduction, suggesting that P. arcticus evolved to grow at low temperatures. Amino acid adaptations and the gene content likely evolved in response to the long-term freezing temperatures (−10°C to −12°C) of the Kolyma (Siberia) permafrost soil from which this strain was isolated. Intracellular water likely does not freeze at these in situ temperatures, which allows P. arcticus to live at subzero temperatures. |
format |
Article in Journal/Newspaper |
author |
Ayala-del-Río, Héctor L. Chain, Patrick S. Grzymski, Joseph J. Ponder, Monica A. Ivanova, Natalia Bergholz, Peter W. Di Bartolo, Genevive Hauser, Loren Land, Miriam Bakermans, Corien Rodrigues, Debora Klappenbach, Joel Zarka, Dan Larimer, Frank Richardson, Paul Murray, Alison Thomashow, Michael Tiedje, James M. |
spellingShingle |
Ayala-del-Río, Héctor L. Chain, Patrick S. Grzymski, Joseph J. Ponder, Monica A. Ivanova, Natalia Bergholz, Peter W. Di Bartolo, Genevive Hauser, Loren Land, Miriam Bakermans, Corien Rodrigues, Debora Klappenbach, Joel Zarka, Dan Larimer, Frank Richardson, Paul Murray, Alison Thomashow, Michael Tiedje, James M. The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth |
author_facet |
Ayala-del-Río, Héctor L. Chain, Patrick S. Grzymski, Joseph J. Ponder, Monica A. Ivanova, Natalia Bergholz, Peter W. Di Bartolo, Genevive Hauser, Loren Land, Miriam Bakermans, Corien Rodrigues, Debora Klappenbach, Joel Zarka, Dan Larimer, Frank Richardson, Paul Murray, Alison Thomashow, Michael Tiedje, James M. |
author_sort |
Ayala-del-Río, Héctor L. |
title |
The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth |
title_short |
The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth |
title_full |
The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth |
title_fullStr |
The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth |
title_full_unstemmed |
The Genome Sequence of Psychrobacter arcticus273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth |
title_sort |
genome sequence of psychrobacter arcticus273-4, a psychroactive siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth |
publisher |
American Society for Microbiology |
publishDate |
2010 |
url |
http://dx.doi.org/10.1128/aem.02101-09 https://journals.asm.org/doi/pdf/10.1128/AEM.02101-09 |
genre |
permafrost Siberia |
genre_facet |
permafrost Siberia |
op_source |
Applied and Environmental Microbiology volume 76, issue 7, page 2304-2312 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.02101-09 |
container_title |
Applied and Environmental Microbiology |
container_volume |
76 |
container_issue |
7 |
container_start_page |
2304 |
op_container_end_page |
2312 |
_version_ |
1810471422974230528 |