Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea
ABSTRACT The South China Sea (SCS), the largest marginal sea in the Western Pacific Ocean, is a huge oligotrophic water body with very limited influx of nitrogenous nutrients. This suggests that sediment microbial N 2 fixation plays an important role in the production of bioavailable nitrogen. To te...
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crasmicro:10.1128/aem.01889-12 2024-09-15T18:18:39+00:00 Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea Dang, Hongyue Yang, Jinying Li, Jing Luan, Xiwu Zhang, Yunbo Gu, Guizhou Xue, Rongrong Zong, Mingyue Klotz, Martin G. 2013 http://dx.doi.org/10.1128/aem.01889-12 https://journals.asm.org/doi/pdf/10.1128/AEM.01889-12 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 79, issue 1, page 121-132 ISSN 0099-2240 1098-5336 journal-article 2013 crasmicro https://doi.org/10.1128/aem.01889-12 2024-09-02T04:05:18Z ABSTRACT The South China Sea (SCS), the largest marginal sea in the Western Pacific Ocean, is a huge oligotrophic water body with very limited influx of nitrogenous nutrients. This suggests that sediment microbial N 2 fixation plays an important role in the production of bioavailable nitrogen. To test the molecular underpinning of this hypothesis, the diversity, abundance, biogeographical distribution, and community structure of the sediment diazotrophic microbiota were investigated at 12 sampling sites, including estuarine, coastal, offshore, deep-sea, and methane hydrate reservoirs or their prospective areas by targeting nifH and some other functional biomarker genes. Diverse and novel nifH sequences were obtained, significantly extending the evolutionary complexity of extant nifH genes. Statistical analyses indicate that sediment in situ temperature is the most significant environmental factor influencing the abundance, community structure, and spatial distribution of the sediment nifH -harboring microbial assemblages in the northern SCS (nSCS). The significantly positive correlation of the sediment pore water NH 4 + concentration with the nifH gene abundance suggests that the nSCS sediment nifH -harboring microbiota is active in N 2 fixation and NH 4 + production. Several other environmental factors, including sediment pore water PO 4 3− concentration, sediment organic carbon, nitrogen and phosphorus levels, etc., are also important in influencing the community structure, spatial distribution, or abundance of the nifH -harboring microbial assemblages. We also confirmed that the nifH genes encoded by archaeal diazotrophs in the ANME-2c subgroup occur exclusively in the deep-sea methane seep areas, providing for the possibility to develop ANME-2c nifH genes as a diagnostic tool for deep-sea methane hydrate reservoir discovery. Article in Journal/Newspaper Methane hydrate ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 79 1 121 132 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
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English |
description |
ABSTRACT The South China Sea (SCS), the largest marginal sea in the Western Pacific Ocean, is a huge oligotrophic water body with very limited influx of nitrogenous nutrients. This suggests that sediment microbial N 2 fixation plays an important role in the production of bioavailable nitrogen. To test the molecular underpinning of this hypothesis, the diversity, abundance, biogeographical distribution, and community structure of the sediment diazotrophic microbiota were investigated at 12 sampling sites, including estuarine, coastal, offshore, deep-sea, and methane hydrate reservoirs or their prospective areas by targeting nifH and some other functional biomarker genes. Diverse and novel nifH sequences were obtained, significantly extending the evolutionary complexity of extant nifH genes. Statistical analyses indicate that sediment in situ temperature is the most significant environmental factor influencing the abundance, community structure, and spatial distribution of the sediment nifH -harboring microbial assemblages in the northern SCS (nSCS). The significantly positive correlation of the sediment pore water NH 4 + concentration with the nifH gene abundance suggests that the nSCS sediment nifH -harboring microbiota is active in N 2 fixation and NH 4 + production. Several other environmental factors, including sediment pore water PO 4 3− concentration, sediment organic carbon, nitrogen and phosphorus levels, etc., are also important in influencing the community structure, spatial distribution, or abundance of the nifH -harboring microbial assemblages. We also confirmed that the nifH genes encoded by archaeal diazotrophs in the ANME-2c subgroup occur exclusively in the deep-sea methane seep areas, providing for the possibility to develop ANME-2c nifH genes as a diagnostic tool for deep-sea methane hydrate reservoir discovery. |
format |
Article in Journal/Newspaper |
author |
Dang, Hongyue Yang, Jinying Li, Jing Luan, Xiwu Zhang, Yunbo Gu, Guizhou Xue, Rongrong Zong, Mingyue Klotz, Martin G. |
spellingShingle |
Dang, Hongyue Yang, Jinying Li, Jing Luan, Xiwu Zhang, Yunbo Gu, Guizhou Xue, Rongrong Zong, Mingyue Klotz, Martin G. Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea |
author_facet |
Dang, Hongyue Yang, Jinying Li, Jing Luan, Xiwu Zhang, Yunbo Gu, Guizhou Xue, Rongrong Zong, Mingyue Klotz, Martin G. |
author_sort |
Dang, Hongyue |
title |
Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea |
title_short |
Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea |
title_full |
Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea |
title_fullStr |
Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea |
title_full_unstemmed |
Environment-Dependent Distribution of the Sediment nifH -Harboring Microbiota in the Northern South China Sea |
title_sort |
environment-dependent distribution of the sediment nifh -harboring microbiota in the northern south china sea |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://dx.doi.org/10.1128/aem.01889-12 https://journals.asm.org/doi/pdf/10.1128/AEM.01889-12 |
genre |
Methane hydrate |
genre_facet |
Methane hydrate |
op_source |
Applied and Environmental Microbiology volume 79, issue 1, page 121-132 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.01889-12 |
container_title |
Applied and Environmental Microbiology |
container_volume |
79 |
container_issue |
1 |
container_start_page |
121 |
op_container_end_page |
132 |
_version_ |
1810456747608899584 |