Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions
ABSTRACT Members of the Roseobacter clade colonize a Spanish turbot larval unit, and one isolate ( Phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. Here, we demonstrate that roseobacters with antagonistic activity against Vibrio anguillarum also colonize a Dani...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2008
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/aem.01738-08 https://journals.asm.org/doi/pdf/10.1128/AEM.01738-08 |
id |
crasmicro:10.1128/aem.01738-08 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/aem.01738-08 2024-09-30T14:42:47+00:00 Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions Porsby, Cisse Hedegaard Nielsen, Kristian Fog Gram, Lone 2008 http://dx.doi.org/10.1128/aem.01738-08 https://journals.asm.org/doi/pdf/10.1128/AEM.01738-08 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 74, issue 23, page 7356-7364 ISSN 0099-2240 1098-5336 journal-article 2008 crasmicro https://doi.org/10.1128/aem.01738-08 2024-09-17T04:08:23Z ABSTRACT Members of the Roseobacter clade colonize a Spanish turbot larval unit, and one isolate ( Phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. Here, we demonstrate that roseobacters with antagonistic activity against Vibrio anguillarum also colonize a Danish turbot larval farm that relies on a very different water source (the Danish fiord Limfjorden as opposed to the Galician Atlantic Ocean). Phylogenetic analyses based on 16S rRNA and gyrase B gene sequences revealed that different species colonized different niches in the larval unit. Phaeobacter inhibens- and Phaeobacter gallaeciensis -like strains were primarily found in the production sites, whereas strains identified as Ruegeria mobilis or Ruegeria pelagia were found only in the algal cultures. Phaeobacter spp. were more inhibitory against the general microbiota from the Danish turbot larval unit than were the Ruegeria spp. Phaeobacter spp. produced tropodithietic acid (TDA) and brown pigment and antagonized V. anguillarum when grown under shaking (200 rpm) and stagnant (0 rpm) conditions, whereas Ruegeria spp. behaved similarly to Phaeobacter strain 27-4 and expressed these three phenotypes only during stagnant growth. Both genera attached to an inert surface and grew in multicellular rosettes after stagnant growth, whereas shaking conditions led to single cells with low attachment capacity. Bacteria from the Roseobacter clade appear to be universal colonizers of marine larval rearing units, and since the Danish Phaeobacter spp. displayed antibacterial activity under a broader range of growth conditions than did Phaeobacter strain 27-4, these organisms may hold greater promise as fish probiotic organisms. Article in Journal/Newspaper Scophthalmus maximus Turbot ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 74 23 7356 7364 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT Members of the Roseobacter clade colonize a Spanish turbot larval unit, and one isolate ( Phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. Here, we demonstrate that roseobacters with antagonistic activity against Vibrio anguillarum also colonize a Danish turbot larval farm that relies on a very different water source (the Danish fiord Limfjorden as opposed to the Galician Atlantic Ocean). Phylogenetic analyses based on 16S rRNA and gyrase B gene sequences revealed that different species colonized different niches in the larval unit. Phaeobacter inhibens- and Phaeobacter gallaeciensis -like strains were primarily found in the production sites, whereas strains identified as Ruegeria mobilis or Ruegeria pelagia were found only in the algal cultures. Phaeobacter spp. were more inhibitory against the general microbiota from the Danish turbot larval unit than were the Ruegeria spp. Phaeobacter spp. produced tropodithietic acid (TDA) and brown pigment and antagonized V. anguillarum when grown under shaking (200 rpm) and stagnant (0 rpm) conditions, whereas Ruegeria spp. behaved similarly to Phaeobacter strain 27-4 and expressed these three phenotypes only during stagnant growth. Both genera attached to an inert surface and grew in multicellular rosettes after stagnant growth, whereas shaking conditions led to single cells with low attachment capacity. Bacteria from the Roseobacter clade appear to be universal colonizers of marine larval rearing units, and since the Danish Phaeobacter spp. displayed antibacterial activity under a broader range of growth conditions than did Phaeobacter strain 27-4, these organisms may hold greater promise as fish probiotic organisms. |
format |
Article in Journal/Newspaper |
author |
Porsby, Cisse Hedegaard Nielsen, Kristian Fog Gram, Lone |
spellingShingle |
Porsby, Cisse Hedegaard Nielsen, Kristian Fog Gram, Lone Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions |
author_facet |
Porsby, Cisse Hedegaard Nielsen, Kristian Fog Gram, Lone |
author_sort |
Porsby, Cisse Hedegaard |
title |
Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions |
title_short |
Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions |
title_full |
Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions |
title_fullStr |
Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions |
title_full_unstemmed |
Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot ( Scophthalmus maximus )-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions |
title_sort |
phaeobacter and ruegeria species of the roseobacter clade colonize separate niches in a danish turbot ( scophthalmus maximus )-rearing farm and antagonize vibrio anguillarum under different growth conditions |
publisher |
American Society for Microbiology |
publishDate |
2008 |
url |
http://dx.doi.org/10.1128/aem.01738-08 https://journals.asm.org/doi/pdf/10.1128/AEM.01738-08 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Applied and Environmental Microbiology volume 74, issue 23, page 7356-7364 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.01738-08 |
container_title |
Applied and Environmental Microbiology |
container_volume |
74 |
container_issue |
23 |
container_start_page |
7356 |
op_container_end_page |
7364 |
_version_ |
1811644648416870400 |