Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis
ABSTRACT In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA f...
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American Society for Microbiology
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Online Access: | http://dx.doi.org/10.1128/aem.01525-09 https://journals.asm.org/doi/pdf/10.1128/AEM.01525-09 |
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crasmicro:10.1128/aem.01525-09 2024-09-15T18:23:22+00:00 Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens 2010 http://dx.doi.org/10.1128/aem.01525-09 https://journals.asm.org/doi/pdf/10.1128/AEM.01525-09 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 76, issue 3, page 776-785 ISSN 0099-2240 1098-5336 journal-article 2010 crasmicro https://doi.org/10.1128/aem.01525-09 2024-08-12T04:06:15Z ABSTRACT In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA , guaA , trpE , purH , glpF , fumC , icd , glyA , and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species. Article in Journal/Newspaper North Atlantic ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 76 3 776 785 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
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English |
description |
ABSTRACT In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA , guaA , trpE , purH , glpF , fumC , icd , glyA , and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species. |
format |
Article in Journal/Newspaper |
author |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens |
spellingShingle |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis |
author_facet |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rosselló-Mora, Ramon Harder, Jens |
author_sort |
Winkelmann, Nadine |
title |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis |
title_short |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis |
title_full |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis |
title_fullStr |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis |
title_full_unstemmed |
Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis |
title_sort |
determination of the diversity of rhodopirellula isolates from european seas by multilocus sequence analysis |
publisher |
American Society for Microbiology |
publishDate |
2010 |
url |
http://dx.doi.org/10.1128/aem.01525-09 https://journals.asm.org/doi/pdf/10.1128/AEM.01525-09 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Applied and Environmental Microbiology volume 76, issue 3, page 776-785 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.01525-09 |
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Applied and Environmental Microbiology |
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76 |
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3 |
container_start_page |
776 |
op_container_end_page |
785 |
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1810463572817346560 |