Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons
ABSTRACT How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined 15 N uptake rate measurements for ammonium, nitrate, and urea with 15 N- and 13...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.01431-14 https://journals.asm.org/doi/pdf/10.1128/AEM.01431-14 |
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crasmicro:10.1128/aem.01431-14 2024-04-28T08:08:06+00:00 Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris Wommack, K. E. 2014 http://dx.doi.org/10.1128/aem.01431-14 https://journals.asm.org/doi/pdf/10.1128/AEM.01431-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 80, issue 19, page 6013-6022 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2014 crasmicro https://doi.org/10.1128/aem.01431-14 2024-04-09T06:52:51Z ABSTRACT How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined 15 N uptake rate measurements for ammonium, nitrate, and urea with 15 N- and 13 C-based DNA stable-isotope probing (SIP). The objective was to identify active bacterial and archeal plankton and their role in N and C uptake during the Arctic summer and winter seasons. We hypothesized that bacteria and archaea would successfully compete for nitrate and urea during the Arctic winter but not during the summer, when phytoplankton dominate the uptake of these nitrogen sources. Samples were collected at a coastal station near Barrow, AK, during August and January. During both seasons, ammonium uptake rates were greater than those for nitrate or urea, and nitrate uptake rates remained lower than those for ammonium or urea. SIP experiments indicated a strong seasonal shift of bacterial and archaeal N utilization from ammonium during the summer to urea during the winter but did not support a similar seasonal pattern of nitrate utilization. Analysis of 16S rRNA gene sequences obtained from each SIP fraction implicated marine group I Crenarchaeota (MGIC) as well as Betaproteobacteria , Firmicutes , SAR11, and SAR324 in N uptake from urea during the winter. Similarly, 13 C SIP data suggested dark carbon fixation for MGIC, as well as for several proteobacterial lineages and the Firmicutes . These data are consistent with urea-fueled nitrification by polar archaea and bacteria, which may be advantageous under dark conditions. Article in Journal/Newspaper Arctic Climate change Phytoplankton ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 80 19 6013 6022 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT How Arctic climate change might translate into alterations of biogeochemical cycles of carbon (C) and nitrogen (N) with respect to inorganic and organic N utilization is not well understood. This study combined 15 N uptake rate measurements for ammonium, nitrate, and urea with 15 N- and 13 C-based DNA stable-isotope probing (SIP). The objective was to identify active bacterial and archeal plankton and their role in N and C uptake during the Arctic summer and winter seasons. We hypothesized that bacteria and archaea would successfully compete for nitrate and urea during the Arctic winter but not during the summer, when phytoplankton dominate the uptake of these nitrogen sources. Samples were collected at a coastal station near Barrow, AK, during August and January. During both seasons, ammonium uptake rates were greater than those for nitrate or urea, and nitrate uptake rates remained lower than those for ammonium or urea. SIP experiments indicated a strong seasonal shift of bacterial and archaeal N utilization from ammonium during the summer to urea during the winter but did not support a similar seasonal pattern of nitrate utilization. Analysis of 16S rRNA gene sequences obtained from each SIP fraction implicated marine group I Crenarchaeota (MGIC) as well as Betaproteobacteria , Firmicutes , SAR11, and SAR324 in N uptake from urea during the winter. Similarly, 13 C SIP data suggested dark carbon fixation for MGIC, as well as for several proteobacterial lineages and the Firmicutes . These data are consistent with urea-fueled nitrification by polar archaea and bacteria, which may be advantageous under dark conditions. |
author2 |
Wommack, K. E. |
format |
Article in Journal/Newspaper |
author |
Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris |
author_facet |
Connelly, Tara L. Baer, Steven E. Cooper, Joshua T. Bronk, Deborah A. Wawrik, Boris |
author_sort |
Connelly, Tara L. |
title |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_short |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_full |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_fullStr |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_full_unstemmed |
Urea Uptake and Carbon Fixation by Marine Pelagic Bacteria and Archaea during the Arctic Summer and Winter Seasons |
title_sort |
urea uptake and carbon fixation by marine pelagic bacteria and archaea during the arctic summer and winter seasons |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://dx.doi.org/10.1128/aem.01431-14 https://journals.asm.org/doi/pdf/10.1128/AEM.01431-14 |
genre |
Arctic Climate change Phytoplankton |
genre_facet |
Arctic Climate change Phytoplankton |
op_source |
Applied and Environmental Microbiology volume 80, issue 19, page 6013-6022 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.01431-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
19 |
container_start_page |
6013 |
op_container_end_page |
6022 |
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1797577001366716416 |