Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales
ABSTRACT Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison ( Bison bison ) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from th...
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Online Access: | http://dx.doi.org/10.1128/aem.01036-12 https://journals.asm.org/doi/pdf/10.1128/AEM.01036-12 |
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crasmicro:10.1128/aem.01036-12 2024-09-15T18:40:46+00:00 Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales Stratilo, Chad W. Bader, Douglas E. 2012 http://dx.doi.org/10.1128/aem.01036-12 https://journals.asm.org/doi/pdf/10.1128/AEM.01036-12 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 78, issue 18, page 6433-6437 ISSN 0099-2240 1098-5336 journal-article 2012 crasmicro https://doi.org/10.1128/aem.01036-12 2024-07-29T04:09:31Z ABSTRACT Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison ( Bison bison ) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time PCR. Confirmed B. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (MLVA15) and single-nucleotide-repeat analysis (SNRA). B. anthracis isolates split into two clades based on MLVA15, while SNRA allowed some isolates between carcass sites to be distinguished from each other. SNRA polymorphisms were also present within a single carcass site. Some isolates from different carcass sites having the same SNRA type had divergent MLVA types; this finding leads to questions about hierarchical typing methods and the robustness of the fine-scale typing of Bacillus anthracis . Article in Journal/Newspaper Wood Buffalo Wood Buffalo National Park Bison bison bison ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 78 18 6433 6437 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison ( Bison bison ) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time PCR. Confirmed B. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (MLVA15) and single-nucleotide-repeat analysis (SNRA). B. anthracis isolates split into two clades based on MLVA15, while SNRA allowed some isolates between carcass sites to be distinguished from each other. SNRA polymorphisms were also present within a single carcass site. Some isolates from different carcass sites having the same SNRA type had divergent MLVA types; this finding leads to questions about hierarchical typing methods and the robustness of the fine-scale typing of Bacillus anthracis . |
format |
Article in Journal/Newspaper |
author |
Stratilo, Chad W. Bader, Douglas E. |
spellingShingle |
Stratilo, Chad W. Bader, Douglas E. Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
author_facet |
Stratilo, Chad W. Bader, Douglas E. |
author_sort |
Stratilo, Chad W. |
title |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_short |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_full |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_fullStr |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_full_unstemmed |
Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales |
title_sort |
genetic diversity among bacillus anthracis soil isolates at fine geographic scales |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://dx.doi.org/10.1128/aem.01036-12 https://journals.asm.org/doi/pdf/10.1128/AEM.01036-12 |
genre |
Wood Buffalo Wood Buffalo National Park Bison bison bison |
genre_facet |
Wood Buffalo Wood Buffalo National Park Bison bison bison |
op_source |
Applied and Environmental Microbiology volume 78, issue 18, page 6433-6437 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.01036-12 |
container_title |
Applied and Environmental Microbiology |
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78 |
container_issue |
18 |
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6433 |
op_container_end_page |
6437 |
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1810485164275400704 |