Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France
ABSTRACT Hepatitis E virus (HEV), an enteric pathogen of both humans and animals, is excreted by infected individuals and is therefore present in wastewaters and coastal waters. As bivalve molluscan shellfish are known to concentrate viral particles during the process of filter feeding, they may acc...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.00978-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00978-14 |
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crasmicro:10.1128/aem.00978-14 2024-09-15T18:03:15+00:00 Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France Grodzki, Marco Schaeffer, Julien Piquet, Jean-Côme Le Saux, Jean-Claude Chevé, Julien Ollivier, Joanna Le Pendu, Jacques Le Guyader, Françoise S. Macfarlane, G. T. 2014 http://dx.doi.org/10.1128/aem.00978-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00978-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 80, issue 14, page 4269-4276 ISSN 0099-2240 1098-5336 journal-article 2014 crasmicro https://doi.org/10.1128/aem.00978-14 2024-07-22T04:09:35Z ABSTRACT Hepatitis E virus (HEV), an enteric pathogen of both humans and animals, is excreted by infected individuals and is therefore present in wastewaters and coastal waters. As bivalve molluscan shellfish are known to concentrate viral particles during the process of filter feeding, they may accumulate this virus. The bioaccumulation efficiencies of oysters ( Crassostrea gigas ), flat oysters ( Ostrea edulis ), mussels ( Mytilus edulis ), and clams ( Ruditapes philippinarum ) were compared at different time points during the year. Tissue distribution analysis showed that most of the viruses were concentrated in the digestive tissues of the four species. Mussels and clams were found to be more sensitive to sporadic contamination events, as demonstrated by rapid bioaccumulation in less than 1 h compared to species of oysters. For oysters, concentrations increased during the 24-h bioaccumulation period. Additionally, to evaluate environmental occurrence of HEV in shellfish, an environmental investigation was undertaken at sites potentially impacted by pigs, wild boars, and human waste. Of the 286 samples collected, none were contaminated with hepatitis E virus, despite evidence that this virus is circulating in some French areas. It is possible that the number of hepatitis E viral particles discharged into the environment is too low to detect or that the virus may have a very short period of persistence in pig manure and human waste. Article in Journal/Newspaper Crassostrea gigas ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 80 14 4269 4276 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Hepatitis E virus (HEV), an enteric pathogen of both humans and animals, is excreted by infected individuals and is therefore present in wastewaters and coastal waters. As bivalve molluscan shellfish are known to concentrate viral particles during the process of filter feeding, they may accumulate this virus. The bioaccumulation efficiencies of oysters ( Crassostrea gigas ), flat oysters ( Ostrea edulis ), mussels ( Mytilus edulis ), and clams ( Ruditapes philippinarum ) were compared at different time points during the year. Tissue distribution analysis showed that most of the viruses were concentrated in the digestive tissues of the four species. Mussels and clams were found to be more sensitive to sporadic contamination events, as demonstrated by rapid bioaccumulation in less than 1 h compared to species of oysters. For oysters, concentrations increased during the 24-h bioaccumulation period. Additionally, to evaluate environmental occurrence of HEV in shellfish, an environmental investigation was undertaken at sites potentially impacted by pigs, wild boars, and human waste. Of the 286 samples collected, none were contaminated with hepatitis E virus, despite evidence that this virus is circulating in some French areas. It is possible that the number of hepatitis E viral particles discharged into the environment is too low to detect or that the virus may have a very short period of persistence in pig manure and human waste. |
author2 |
Macfarlane, G. T. |
format |
Article in Journal/Newspaper |
author |
Grodzki, Marco Schaeffer, Julien Piquet, Jean-Côme Le Saux, Jean-Claude Chevé, Julien Ollivier, Joanna Le Pendu, Jacques Le Guyader, Françoise S. |
spellingShingle |
Grodzki, Marco Schaeffer, Julien Piquet, Jean-Côme Le Saux, Jean-Claude Chevé, Julien Ollivier, Joanna Le Pendu, Jacques Le Guyader, Françoise S. Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France |
author_facet |
Grodzki, Marco Schaeffer, Julien Piquet, Jean-Côme Le Saux, Jean-Claude Chevé, Julien Ollivier, Joanna Le Pendu, Jacques Le Guyader, Françoise S. |
author_sort |
Grodzki, Marco |
title |
Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France |
title_short |
Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France |
title_full |
Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France |
title_fullStr |
Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France |
title_full_unstemmed |
Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France |
title_sort |
bioaccumulation efficiency, tissue distribution, and environmental occurrence of hepatitis e virus in bivalve shellfish from france |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://dx.doi.org/10.1128/aem.00978-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00978-14 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Applied and Environmental Microbiology volume 80, issue 14, page 4269-4276 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00978-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
14 |
container_start_page |
4269 |
op_container_end_page |
4276 |
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1810440769036615680 |