Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces
ABSTRACT Psychrobacter arcticus strain 273-4, an isolate from a Siberian permafrost core, is capable of forming biofilms when grown in minimal medium under laboratory conditions. Biofilms form at 4 to 22°C when acetate is supplied as the lone carbon source and with 1 to 7% sea salt. P. arcticus is a...
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Online Access: | http://dx.doi.org/10.1128/aem.00867-13 https://journals.asm.org/doi/pdf/10.1128/AEM.00867-13 |
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crasmicro:10.1128/aem.00867-13 2024-05-19T07:47:12+00:00 Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces Hinsa-Leasure, Shannon M. Koid, Cassandra Tiedje, James M. Schultzhaus, Janna N. 2013 http://dx.doi.org/10.1128/aem.00867-13 https://journals.asm.org/doi/pdf/10.1128/AEM.00867-13 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 79, issue 13, page 3967-3973 ISSN 0099-2240 1098-5336 journal-article 2013 crasmicro https://doi.org/10.1128/aem.00867-13 2024-04-25T06:50:09Z ABSTRACT Psychrobacter arcticus strain 273-4, an isolate from a Siberian permafrost core, is capable of forming biofilms when grown in minimal medium under laboratory conditions. Biofilms form at 4 to 22°C when acetate is supplied as the lone carbon source and with 1 to 7% sea salt. P. arcticus is also capable of colonizing quartz sand. Transposon mutagenesis identified a gene important for biofilm formation by P. arcticus . Four transposon mutants were mapped to a 20.1-kbp gene, which is predicted to encode a protein of 6,715 amino acids (Psyc_1601). We refer to this open reading frame as cat1 , for cold attachment gene 1. The cat1 mutants are unable to form biofilms at levels equivalent to that of the wild type, and there is no impact on the planktonic growth characteristics of the strains, indicating a specific role in biofilm formation. Through time course studies of the static microtiter plate assay, we determined that cat1 mutants are unable to form biofilms equivalent to that of the wild type under all conditions tested. In flow cell experiments, cat1 mutants initially are unable to attach to the surface. Over time, however, they form microcolonies, an architecture very different from that produced by wild-type biofilms. Our results demonstrate that Cat1 is involved in the initial stages of bacterial attachment to surfaces. Article in Journal/Newspaper permafrost ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 79 13 3967 3973 |
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ASM Journals (American Society for Microbiology) |
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English |
description |
ABSTRACT Psychrobacter arcticus strain 273-4, an isolate from a Siberian permafrost core, is capable of forming biofilms when grown in minimal medium under laboratory conditions. Biofilms form at 4 to 22°C when acetate is supplied as the lone carbon source and with 1 to 7% sea salt. P. arcticus is also capable of colonizing quartz sand. Transposon mutagenesis identified a gene important for biofilm formation by P. arcticus . Four transposon mutants were mapped to a 20.1-kbp gene, which is predicted to encode a protein of 6,715 amino acids (Psyc_1601). We refer to this open reading frame as cat1 , for cold attachment gene 1. The cat1 mutants are unable to form biofilms at levels equivalent to that of the wild type, and there is no impact on the planktonic growth characteristics of the strains, indicating a specific role in biofilm formation. Through time course studies of the static microtiter plate assay, we determined that cat1 mutants are unable to form biofilms equivalent to that of the wild type under all conditions tested. In flow cell experiments, cat1 mutants initially are unable to attach to the surface. Over time, however, they form microcolonies, an architecture very different from that produced by wild-type biofilms. Our results demonstrate that Cat1 is involved in the initial stages of bacterial attachment to surfaces. |
format |
Article in Journal/Newspaper |
author |
Hinsa-Leasure, Shannon M. Koid, Cassandra Tiedje, James M. Schultzhaus, Janna N. |
spellingShingle |
Hinsa-Leasure, Shannon M. Koid, Cassandra Tiedje, James M. Schultzhaus, Janna N. Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces |
author_facet |
Hinsa-Leasure, Shannon M. Koid, Cassandra Tiedje, James M. Schultzhaus, Janna N. |
author_sort |
Hinsa-Leasure, Shannon M. |
title |
Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces |
title_short |
Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces |
title_full |
Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces |
title_fullStr |
Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces |
title_full_unstemmed |
Biofilm Formation by Psychrobacter arcticus and the Role of a Large Adhesin in Attachment to Surfaces |
title_sort |
biofilm formation by psychrobacter arcticus and the role of a large adhesin in attachment to surfaces |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://dx.doi.org/10.1128/aem.00867-13 https://journals.asm.org/doi/pdf/10.1128/AEM.00867-13 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Applied and Environmental Microbiology volume 79, issue 13, page 3967-3973 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00867-13 |
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Applied and Environmental Microbiology |
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79 |
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13 |
container_start_page |
3967 |
op_container_end_page |
3973 |
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1799487539602522112 |