Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes
ABSTRACT Methane (CH 4 ) flux to the atmosphere is mitigated via microbial CH 4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH 4 em...
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Online Access: | http://dx.doi.org/10.1128/aem.00853-12 https://journals.asm.org/doi/pdf/10.1128/AEM.00853-12 |
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crasmicro:10.1128/aem.00853-12 2024-09-15T18:25:04+00:00 Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth 2012 http://dx.doi.org/10.1128/aem.00853-12 https://journals.asm.org/doi/pdf/10.1128/AEM.00853-12 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 78, issue 13, page 4715-4723 ISSN 0099-2240 1098-5336 journal-article 2012 crasmicro https://doi.org/10.1128/aem.00853-12 2024-08-19T04:06:13Z ABSTRACT Methane (CH 4 ) flux to the atmosphere is mitigated via microbial CH 4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH 4 emissions. We used DNA-based stable-isotope probing (SIP), quantitative PCR (Q-PCR), and pyrosequencing analyses to identify and characterize methanotrophic communities active at a range of temperatures (4°C, 10°C, and 21°C) in sediments (to a depth of 25 cm) sampled from Lake Qalluuraq on the North Slope of Alaska. CH 4 oxidation activity was measured in microcosm incubations containing sediments at all temperatures, with the highest CH 4 oxidation potential of 37.5 μmol g −1 day −1 in the uppermost (depth, 0 to 1 cm) sediment at 21°C after 2 to 5 days of incubation. Q-PCR of pmoA and of the 16S rRNA genes of type I and type II methanotrophs, and pyrosequencing of 16S rRNA genes in 13 C-labeled DNA obtained by SIP demonstrated that the type I methanotrophs Methylobacter , Methylomonas , and Methylosoma dominated carbon acquisition from CH 4 in the sediments. The identity and relative abundance of active methanotrophs differed with the incubation temperature. Methylotrophs were also abundant in the microbial community that derived carbon from CH 4 , especially in the deeper sediments (depth, 15 to 20 cm) at low temperatures (4°C and 10°C), and showed a good linear relationship ( R = 0.82) with the relative abundances of methanotrophs in pyrosequencing reads. This study describes for the first time how methanotrophic communities in arctic lake sediments respond to temperature variations. Article in Journal/Newspaper north slope Alaska ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 78 13 4715 4723 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Methane (CH 4 ) flux to the atmosphere is mitigated via microbial CH 4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH 4 emissions. We used DNA-based stable-isotope probing (SIP), quantitative PCR (Q-PCR), and pyrosequencing analyses to identify and characterize methanotrophic communities active at a range of temperatures (4°C, 10°C, and 21°C) in sediments (to a depth of 25 cm) sampled from Lake Qalluuraq on the North Slope of Alaska. CH 4 oxidation activity was measured in microcosm incubations containing sediments at all temperatures, with the highest CH 4 oxidation potential of 37.5 μmol g −1 day −1 in the uppermost (depth, 0 to 1 cm) sediment at 21°C after 2 to 5 days of incubation. Q-PCR of pmoA and of the 16S rRNA genes of type I and type II methanotrophs, and pyrosequencing of 16S rRNA genes in 13 C-labeled DNA obtained by SIP demonstrated that the type I methanotrophs Methylobacter , Methylomonas , and Methylosoma dominated carbon acquisition from CH 4 in the sediments. The identity and relative abundance of active methanotrophs differed with the incubation temperature. Methylotrophs were also abundant in the microbial community that derived carbon from CH 4 , especially in the deeper sediments (depth, 15 to 20 cm) at low temperatures (4°C and 10°C), and showed a good linear relationship ( R = 0.82) with the relative abundances of methanotrophs in pyrosequencing reads. This study describes for the first time how methanotrophic communities in arctic lake sediments respond to temperature variations. |
format |
Article in Journal/Newspaper |
author |
He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth |
spellingShingle |
He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
author_facet |
He, Ruo Wooller, Matthew J. Pohlman, John W. Quensen, John Tiedje, James M. Leigh, Mary Beth |
author_sort |
He, Ruo |
title |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_short |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_full |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_fullStr |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_full_unstemmed |
Shifts in Identity and Activity of Methanotrophs in Arctic Lake Sediments in Response to Temperature Changes |
title_sort |
shifts in identity and activity of methanotrophs in arctic lake sediments in response to temperature changes |
publisher |
American Society for Microbiology |
publishDate |
2012 |
url |
http://dx.doi.org/10.1128/aem.00853-12 https://journals.asm.org/doi/pdf/10.1128/AEM.00853-12 |
genre |
north slope Alaska |
genre_facet |
north slope Alaska |
op_source |
Applied and Environmental Microbiology volume 78, issue 13, page 4715-4723 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00853-12 |
container_title |
Applied and Environmental Microbiology |
container_volume |
78 |
container_issue |
13 |
container_start_page |
4715 |
op_container_end_page |
4723 |
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1810465480631123968 |