Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin
ABSTRACT Photobacterium damselae subsp. damselae is considered to be an emerging pathogen of marine fish of importance in aquaculture, with a notable increase in its geographical distribution during the last several years. In this study, we carried out for the first time to our knowledge a genetic a...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2016
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/aem.00781-16 https://journals.asm.org/doi/pdf/10.1128/AEM.00781-16 |
id |
crasmicro:10.1128/aem.00781-16 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/aem.00781-16 2024-06-23T07:57:20+00:00 Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin Terceti, Mateus S. Ogut, Hamdi Osorio, Carlos R. Björkroth, J. 2016 http://dx.doi.org/10.1128/aem.00781-16 https://journals.asm.org/doi/pdf/10.1128/AEM.00781-16 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 82, issue 13, page 3736-3745 ISSN 0099-2240 1098-5336 journal-article 2016 crasmicro https://doi.org/10.1128/aem.00781-16 2024-06-10T04:07:35Z ABSTRACT Photobacterium damselae subsp. damselae is considered to be an emerging pathogen of marine fish of importance in aquaculture, with a notable increase in its geographical distribution during the last several years. In this study, we carried out for the first time to our knowledge a genetic and pathobiological characterization of 14 strains isolated from sea bass ( Dicentrarchus labrax ) reared in the Southeastern Black Sea, where high mortalities were observed at two aquaculture farms during the summer and autumn of 2011. Heterogeneity was evidenced among strains in phenotypical traits, such as sucrose fermentation, motility, and hemolysis. Although 11 of 14 isolates were hemolytic, we found that all of the isolates lacked the pPHDD1 virulence plasmid that encodes the phospholipase-D damselysin (Dly) and the pore-forming toxin PhlyP, two hemolysins previously reported to constitute major virulence factors for turbot. Subsequent PCR and sequencing analyses demonstrated that the 11 hemolytic isolates harbored a complete hlyA ch gene, a chromosome I-borne gene that encodes HlyA ch hemolysin, whereas the three nonhemolytic isolates contained hlyA ch pseudogenes caused by insertion sequence elements. Virulence challenges with two representative strains revealed that, albeit less virulent than the pPHDD1-harboring strain RM-71, the plasmidless hlyA ch -positive and hlyA ch -negative Black Sea isolates were pathogenic for sea bass. A phylogenetic analysis based on the toxR gene sequence uncovered a greater diversity in the isolates, indicating that the presence of this pathogen in the Black Sea was not caused by the introduction and spread of a single virulent clone but by the proliferation of different clones. IMPORTANCE The geographical distribution of marine bacterial pathogens is undergoing a worldwide increase. In particular, bacteria of the group vibrios are increasingly being isolated as the causative agents of disease in novel species of cultivated fish in areas where they had not been previously ... Article in Journal/Newspaper Turbot ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 82 13 3736 3745 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
description |
ABSTRACT Photobacterium damselae subsp. damselae is considered to be an emerging pathogen of marine fish of importance in aquaculture, with a notable increase in its geographical distribution during the last several years. In this study, we carried out for the first time to our knowledge a genetic and pathobiological characterization of 14 strains isolated from sea bass ( Dicentrarchus labrax ) reared in the Southeastern Black Sea, where high mortalities were observed at two aquaculture farms during the summer and autumn of 2011. Heterogeneity was evidenced among strains in phenotypical traits, such as sucrose fermentation, motility, and hemolysis. Although 11 of 14 isolates were hemolytic, we found that all of the isolates lacked the pPHDD1 virulence plasmid that encodes the phospholipase-D damselysin (Dly) and the pore-forming toxin PhlyP, two hemolysins previously reported to constitute major virulence factors for turbot. Subsequent PCR and sequencing analyses demonstrated that the 11 hemolytic isolates harbored a complete hlyA ch gene, a chromosome I-borne gene that encodes HlyA ch hemolysin, whereas the three nonhemolytic isolates contained hlyA ch pseudogenes caused by insertion sequence elements. Virulence challenges with two representative strains revealed that, albeit less virulent than the pPHDD1-harboring strain RM-71, the plasmidless hlyA ch -positive and hlyA ch -negative Black Sea isolates were pathogenic for sea bass. A phylogenetic analysis based on the toxR gene sequence uncovered a greater diversity in the isolates, indicating that the presence of this pathogen in the Black Sea was not caused by the introduction and spread of a single virulent clone but by the proliferation of different clones. IMPORTANCE The geographical distribution of marine bacterial pathogens is undergoing a worldwide increase. In particular, bacteria of the group vibrios are increasingly being isolated as the causative agents of disease in novel species of cultivated fish in areas where they had not been previously ... |
author2 |
Björkroth, J. |
format |
Article in Journal/Newspaper |
author |
Terceti, Mateus S. Ogut, Hamdi Osorio, Carlos R. |
spellingShingle |
Terceti, Mateus S. Ogut, Hamdi Osorio, Carlos R. Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin |
author_facet |
Terceti, Mateus S. Ogut, Hamdi Osorio, Carlos R. |
author_sort |
Terceti, Mateus S. |
title |
Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin |
title_short |
Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin |
title_full |
Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin |
title_fullStr |
Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin |
title_full_unstemmed |
Photobacterium damselae subsp. damselae, an Emerging Fish Pathogen in the Black Sea: Evidence of a Multiclonal Origin |
title_sort |
photobacterium damselae subsp. damselae, an emerging fish pathogen in the black sea: evidence of a multiclonal origin |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
http://dx.doi.org/10.1128/aem.00781-16 https://journals.asm.org/doi/pdf/10.1128/AEM.00781-16 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Applied and Environmental Microbiology volume 82, issue 13, page 3736-3745 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00781-16 |
container_title |
Applied and Environmental Microbiology |
container_volume |
82 |
container_issue |
13 |
container_start_page |
3736 |
op_container_end_page |
3745 |
_version_ |
1802650927796060160 |