Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice
ABSTRACT Proteorhodopsins (PRs) are widespread bacterial integral membrane proteins that function as light-driven proton pumps. Antarctic sea ice supports a complex community of autotrophic algae, heterotrophic bacteria, viruses, and protists that are an important food source for higher trophic leve...
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Online Access: | http://dx.doi.org/10.1128/aem.00562-10 https://journals.asm.org/doi/pdf/10.1128/AEM.00562-10 |
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crasmicro:10.1128/aem.00562-10 2024-06-23T07:46:47+00:00 Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice Koh, Eileen Y. Atamna-Ismaeel, Nof Martin, Andrew Cowie, Rebecca O. M. Beja, Oded Davy, Simon K. Maas, Elizabeth W. Ryan, Ken G. 2010 http://dx.doi.org/10.1128/aem.00562-10 https://journals.asm.org/doi/pdf/10.1128/AEM.00562-10 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 76, issue 17, page 5918-5925 ISSN 0099-2240 1098-5336 journal-article 2010 crasmicro https://doi.org/10.1128/aem.00562-10 2024-06-03T08:11:00Z ABSTRACT Proteorhodopsins (PRs) are widespread bacterial integral membrane proteins that function as light-driven proton pumps. Antarctic sea ice supports a complex community of autotrophic algae, heterotrophic bacteria, viruses, and protists that are an important food source for higher trophic levels in ice-covered regions of the Southern Ocean. Here, we present the first report of PR-bearing bacteria, both dormant and active, in Antarctic sea ice from a series of sites in the Ross Sea using gene-specific primers. Positive PR sequences were generated from genomic DNA at all depths in sea ice, and these sequences aligned with the classes Alphaproteobacteria , Gammaproteobacteria , and Flavobacteria . The sequences showed some similarity to previously reported PR sequences, although most of the sequences were generally distinct. Positive PR sequences were also observed from cDNA reverse transcribed from RNA isolated from sea ice samples. This finding indicates that these sequences were generated from metabolically active cells and suggests that the PR gene is functional within sea ice. Both blue-absorbing and green-absorbing forms of PRs were detected, and only a limited number of blue-absorbing forms were found and were in the midsection of the sea ice profile in this study. Questions still remain regarding the protein's ecological functions, and ultimately, field experiments will be needed to establish the ecological and functional role of PRs in the sea ice ecosystem. Article in Journal/Newspaper Antarc* Antarctic Ross Sea Sea ice Southern Ocean ASM Journals (American Society for Microbiology) Antarctic Ross Sea Southern Ocean Applied and Environmental Microbiology 76 17 5918 5925 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
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English |
description |
ABSTRACT Proteorhodopsins (PRs) are widespread bacterial integral membrane proteins that function as light-driven proton pumps. Antarctic sea ice supports a complex community of autotrophic algae, heterotrophic bacteria, viruses, and protists that are an important food source for higher trophic levels in ice-covered regions of the Southern Ocean. Here, we present the first report of PR-bearing bacteria, both dormant and active, in Antarctic sea ice from a series of sites in the Ross Sea using gene-specific primers. Positive PR sequences were generated from genomic DNA at all depths in sea ice, and these sequences aligned with the classes Alphaproteobacteria , Gammaproteobacteria , and Flavobacteria . The sequences showed some similarity to previously reported PR sequences, although most of the sequences were generally distinct. Positive PR sequences were also observed from cDNA reverse transcribed from RNA isolated from sea ice samples. This finding indicates that these sequences were generated from metabolically active cells and suggests that the PR gene is functional within sea ice. Both blue-absorbing and green-absorbing forms of PRs were detected, and only a limited number of blue-absorbing forms were found and were in the midsection of the sea ice profile in this study. Questions still remain regarding the protein's ecological functions, and ultimately, field experiments will be needed to establish the ecological and functional role of PRs in the sea ice ecosystem. |
format |
Article in Journal/Newspaper |
author |
Koh, Eileen Y. Atamna-Ismaeel, Nof Martin, Andrew Cowie, Rebecca O. M. Beja, Oded Davy, Simon K. Maas, Elizabeth W. Ryan, Ken G. |
spellingShingle |
Koh, Eileen Y. Atamna-Ismaeel, Nof Martin, Andrew Cowie, Rebecca O. M. Beja, Oded Davy, Simon K. Maas, Elizabeth W. Ryan, Ken G. Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice |
author_facet |
Koh, Eileen Y. Atamna-Ismaeel, Nof Martin, Andrew Cowie, Rebecca O. M. Beja, Oded Davy, Simon K. Maas, Elizabeth W. Ryan, Ken G. |
author_sort |
Koh, Eileen Y. |
title |
Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice |
title_short |
Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice |
title_full |
Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice |
title_fullStr |
Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice |
title_full_unstemmed |
Proteorhodopsin-Bearing Bacteria in Antarctic Sea Ice |
title_sort |
proteorhodopsin-bearing bacteria in antarctic sea ice |
publisher |
American Society for Microbiology |
publishDate |
2010 |
url |
http://dx.doi.org/10.1128/aem.00562-10 https://journals.asm.org/doi/pdf/10.1128/AEM.00562-10 |
geographic |
Antarctic Ross Sea Southern Ocean |
geographic_facet |
Antarctic Ross Sea Southern Ocean |
genre |
Antarc* Antarctic Ross Sea Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Ross Sea Sea ice Southern Ocean |
op_source |
Applied and Environmental Microbiology volume 76, issue 17, page 5918-5925 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00562-10 |
container_title |
Applied and Environmental Microbiology |
container_volume |
76 |
container_issue |
17 |
container_start_page |
5918 |
op_container_end_page |
5925 |
_version_ |
1802648162918203392 |