Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost
Understanding the long-term survivability and associated metabolic traits of microorganisms in ancient permafrost frozen millions of years ago provides a unique window into the burial and preservation processes experienced in general by subsurface microorganisms in sedimentary deposits because of pe...
Published in: | Applied and Environmental Microbiology |
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Language: | English |
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American Society for Microbiology
2019
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Online Access: | http://dx.doi.org/10.1128/aem.00560-19 https://journals.asm.org/doi/pdf/10.1128/AEM.00560-19 |
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crasmicro:10.1128/aem.00560-19 2024-06-23T07:56:02+00:00 Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost Liang, Renxing Lau, Maggie Vishnivetskaya, Tatiana Lloyd, Karen G. Wang, Wei Wiggins, Jessica Miller, Jennifer Pfiffner, Susan Rivkina, Elizaveta M. Onstott, Tullis C. Stams, Alfons J. M. Russian Government Assignment Russian Government Assignment National Science Foundation (NSF), Office Of International Science & Engineering National Science Foundation National Science Foundation National Science Foundation 2019 http://dx.doi.org/10.1128/aem.00560-19 https://journals.asm.org/doi/pdf/10.1128/AEM.00560-19 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 85, issue 15 ISSN 0099-2240 1098-5336 journal-article 2019 crasmicro https://doi.org/10.1128/aem.00560-19 2024-05-27T13:00:04Z Understanding the long-term survivability and associated metabolic traits of microorganisms in ancient permafrost frozen millions of years ago provides a unique window into the burial and preservation processes experienced in general by subsurface microorganisms in sedimentary deposits because of permafrost’s hydrological isolation and exceptional DNA preservation. We employed aspartic acid racemization modeling and metagenomics to determine which microbial communities were metabolically active in the 1.1-Ma permafrost from northeastern Siberia. The simultaneous sequencing of extracellular and intracellular genomic DNA provided insight into the metabolic potential distinguishing extinct from extant microorganisms under frozen conditions over this time interval. This in-depth metagenomic sequencing advances our understanding of the microbial diversity and metabolic functions of extant microbiomes from early Pleistocene permafrost. Therefore, these findings extend our knowledge of the survivability of microbes in permafrost from 33,000 years to 1.1 Ma. Article in Journal/Newspaper permafrost Siberia ASM Journals (American Society for Microbiology) Applied and Environmental Microbiology 85 15 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
Understanding the long-term survivability and associated metabolic traits of microorganisms in ancient permafrost frozen millions of years ago provides a unique window into the burial and preservation processes experienced in general by subsurface microorganisms in sedimentary deposits because of permafrost’s hydrological isolation and exceptional DNA preservation. We employed aspartic acid racemization modeling and metagenomics to determine which microbial communities were metabolically active in the 1.1-Ma permafrost from northeastern Siberia. The simultaneous sequencing of extracellular and intracellular genomic DNA provided insight into the metabolic potential distinguishing extinct from extant microorganisms under frozen conditions over this time interval. This in-depth metagenomic sequencing advances our understanding of the microbial diversity and metabolic functions of extant microbiomes from early Pleistocene permafrost. Therefore, these findings extend our knowledge of the survivability of microbes in permafrost from 33,000 years to 1.1 Ma. |
author2 |
Stams, Alfons J. M. Russian Government Assignment Russian Government Assignment National Science Foundation (NSF), Office Of International Science & Engineering National Science Foundation National Science Foundation National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Liang, Renxing Lau, Maggie Vishnivetskaya, Tatiana Lloyd, Karen G. Wang, Wei Wiggins, Jessica Miller, Jennifer Pfiffner, Susan Rivkina, Elizaveta M. Onstott, Tullis C. |
spellingShingle |
Liang, Renxing Lau, Maggie Vishnivetskaya, Tatiana Lloyd, Karen G. Wang, Wei Wiggins, Jessica Miller, Jennifer Pfiffner, Susan Rivkina, Elizaveta M. Onstott, Tullis C. Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost |
author_facet |
Liang, Renxing Lau, Maggie Vishnivetskaya, Tatiana Lloyd, Karen G. Wang, Wei Wiggins, Jessica Miller, Jennifer Pfiffner, Susan Rivkina, Elizaveta M. Onstott, Tullis C. |
author_sort |
Liang, Renxing |
title |
Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost |
title_short |
Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost |
title_full |
Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost |
title_fullStr |
Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost |
title_full_unstemmed |
Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost |
title_sort |
predominance of anaerobic, spore-forming bacteria in metabolically active microbial communities from ancient siberian permafrost |
publisher |
American Society for Microbiology |
publishDate |
2019 |
url |
http://dx.doi.org/10.1128/aem.00560-19 https://journals.asm.org/doi/pdf/10.1128/AEM.00560-19 |
genre |
permafrost Siberia |
genre_facet |
permafrost Siberia |
op_source |
Applied and Environmental Microbiology volume 85, issue 15 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00560-19 |
container_title |
Applied and Environmental Microbiology |
container_volume |
85 |
container_issue |
15 |
_version_ |
1802648889806815232 |