Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality
ABSTRACT A number of bivalve species worldwide, including the Pacific oyster, Crassostrea gigas , have been affected by mass mortality events associated with herpesviruses, resulting in significant losses. A particular herpesvirus was purified from naturally infected larval Pacific oysters, and its...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.00484-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00484-14 |
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crasmicro:10.1128/aem.00484-14 2024-06-23T07:52:16+00:00 Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality Renault, Tristan Bouquet, Anne Lise Maurice, Julien-Thomas Lupo, Coralie Blachier, Philippe Wommack, K. E. 2014 http://dx.doi.org/10.1128/aem.00484-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00484-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 80, issue 17, page 5419-5426 ISSN 0099-2240 1098-5336 journal-article 2014 crasmicro https://doi.org/10.1128/aem.00484-14 2024-06-03T08:10:37Z ABSTRACT A number of bivalve species worldwide, including the Pacific oyster, Crassostrea gigas , have been affected by mass mortality events associated with herpesviruses, resulting in significant losses. A particular herpesvirus was purified from naturally infected larval Pacific oysters, and its genome was completely sequenced. This virus has been classified as Ostreid herpesvirus 1 (OsHV-1) within the family Malacoherpesviridae . Since 2008, mass mortality outbreaks among C. gigas in Europe have been related to the detection of a variant of OsHV-1 called μVar. Additional data are necessary to better describe mortality events in relation to environmental-parameter fluctuations and OsHV-1 detection. For this purpose, a single batch of Pacific oyster spat was deployed in 4 different locations in the Marennes-Oleron area (France): an oyster pond (“claire”), a shellfish nursery, and two locations in the field. Mortality rates were recorded based on regular observation, and samples were collected to search for and quantify OsHV-1 DNA by real-time PCR. Although similar massive mortality rates were reported at the 4 sites, mortality was detected earlier in the pond and in the nursery than at both field sites. This difference may be related to earlier increases in water temperature. Mass mortality was observed among oysters a few days after increases in the number of PCR-positive oysters and viral-DNA amounts were recorded. An initial increment in the number of PCR-positive oysters was reported at both field sites during the survey in the absence of significant mortality. During this period, the water temperature was below 16°C. Article in Journal/Newspaper Crassostrea gigas Pacific oyster ASM Journals (American Society for Microbiology) Pacific Applied and Environmental Microbiology 80 17 5419 5426 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT A number of bivalve species worldwide, including the Pacific oyster, Crassostrea gigas , have been affected by mass mortality events associated with herpesviruses, resulting in significant losses. A particular herpesvirus was purified from naturally infected larval Pacific oysters, and its genome was completely sequenced. This virus has been classified as Ostreid herpesvirus 1 (OsHV-1) within the family Malacoherpesviridae . Since 2008, mass mortality outbreaks among C. gigas in Europe have been related to the detection of a variant of OsHV-1 called μVar. Additional data are necessary to better describe mortality events in relation to environmental-parameter fluctuations and OsHV-1 detection. For this purpose, a single batch of Pacific oyster spat was deployed in 4 different locations in the Marennes-Oleron area (France): an oyster pond (“claire”), a shellfish nursery, and two locations in the field. Mortality rates were recorded based on regular observation, and samples were collected to search for and quantify OsHV-1 DNA by real-time PCR. Although similar massive mortality rates were reported at the 4 sites, mortality was detected earlier in the pond and in the nursery than at both field sites. This difference may be related to earlier increases in water temperature. Mass mortality was observed among oysters a few days after increases in the number of PCR-positive oysters and viral-DNA amounts were recorded. An initial increment in the number of PCR-positive oysters was reported at both field sites during the survey in the absence of significant mortality. During this period, the water temperature was below 16°C. |
author2 |
Wommack, K. E. |
format |
Article in Journal/Newspaper |
author |
Renault, Tristan Bouquet, Anne Lise Maurice, Julien-Thomas Lupo, Coralie Blachier, Philippe |
spellingShingle |
Renault, Tristan Bouquet, Anne Lise Maurice, Julien-Thomas Lupo, Coralie Blachier, Philippe Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality |
author_facet |
Renault, Tristan Bouquet, Anne Lise Maurice, Julien-Thomas Lupo, Coralie Blachier, Philippe |
author_sort |
Renault, Tristan |
title |
Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality |
title_short |
Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality |
title_full |
Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality |
title_fullStr |
Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality |
title_full_unstemmed |
Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality |
title_sort |
ostreid herpesvirus 1 infection among pacific oyster (crassostrea gigas) spat: relevance of water temperature to virus replication and circulation prior to the onset of mortality |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://dx.doi.org/10.1128/aem.00484-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00484-14 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Applied and Environmental Microbiology volume 80, issue 17, page 5419-5426 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00484-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
17 |
container_start_page |
5419 |
op_container_end_page |
5426 |
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1802643534760640512 |