Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining
ABSTRACT Biovolume is an important characteristic of cells that shapes the contribution of microbes to total biomass and biogeochemical cycling. Most studies of bacterial cell volumes use DAPI (4′,6′-diamidino-2-phenylindole), which stains nucleic acids and therefore only a portion of the cell. We u...
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crasmicro:10.1128/aem.00183-09 2023-11-05T03:36:57+01:00 Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining Straza, Tiffany R. A. Cottrell, Matthew T. Ducklow, Hugh W. Kirchman, David L. 2009 http://dx.doi.org/10.1128/aem.00183-09 https://journals.asm.org/doi/pdf/10.1128/AEM.00183-09 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 75, issue 12, page 4028-4034 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2009 crasmicro https://doi.org/10.1128/aem.00183-09 2023-10-09T16:04:42Z ABSTRACT Biovolume is an important characteristic of cells that shapes the contribution of microbes to total biomass and biogeochemical cycling. Most studies of bacterial cell volumes use DAPI (4′,6′-diamidino-2-phenylindole), which stains nucleic acids and therefore only a portion of the cell. We used SYPRO Ruby protein stain combined with fluorescence in situ hybridization to examine biovolumes of bacteria in the total community, as well in phylogenetic subgroups. Protein-based volumes varied more and were consistently larger than DNA-based volumes by 3.3-fold on average. Bacterial cells were ca. 30% larger in the Arctic Ocean and Antarctic coastal waters than in temperate regimes. We hypothesized that geographic differences in the abundance of specific bacterial groups drove the observed patterns in biovolume. In support of this hypothesis, we found that Gammaproteobacteria and members of the Sphingobacteria-Flavobacteria group were larger in higher-latitude waters and that the mean volumes of both groups were larger than the mean bacterial volume in all environments tested. The mean cell size of SAR11 bacteria was larger than the mean cell size of the total bacterial community on average, although this varied. Protein staining increases the accuracy of biovolume measurements and gives insights into how the biomass of marine microbial communities varies over time and space. Article in Journal/Newspaper Antarc* Antarctic Arctic Arctic Ocean ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 75 12 4028 4034 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Straza, Tiffany R. A. Cottrell, Matthew T. Ducklow, Hugh W. Kirchman, David L. Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Biovolume is an important characteristic of cells that shapes the contribution of microbes to total biomass and biogeochemical cycling. Most studies of bacterial cell volumes use DAPI (4′,6′-diamidino-2-phenylindole), which stains nucleic acids and therefore only a portion of the cell. We used SYPRO Ruby protein stain combined with fluorescence in situ hybridization to examine biovolumes of bacteria in the total community, as well in phylogenetic subgroups. Protein-based volumes varied more and were consistently larger than DNA-based volumes by 3.3-fold on average. Bacterial cells were ca. 30% larger in the Arctic Ocean and Antarctic coastal waters than in temperate regimes. We hypothesized that geographic differences in the abundance of specific bacterial groups drove the observed patterns in biovolume. In support of this hypothesis, we found that Gammaproteobacteria and members of the Sphingobacteria-Flavobacteria group were larger in higher-latitude waters and that the mean volumes of both groups were larger than the mean bacterial volume in all environments tested. The mean cell size of SAR11 bacteria was larger than the mean cell size of the total bacterial community on average, although this varied. Protein staining increases the accuracy of biovolume measurements and gives insights into how the biomass of marine microbial communities varies over time and space. |
format |
Article in Journal/Newspaper |
author |
Straza, Tiffany R. A. Cottrell, Matthew T. Ducklow, Hugh W. Kirchman, David L. |
author_facet |
Straza, Tiffany R. A. Cottrell, Matthew T. Ducklow, Hugh W. Kirchman, David L. |
author_sort |
Straza, Tiffany R. A. |
title |
Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining |
title_short |
Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining |
title_full |
Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining |
title_fullStr |
Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining |
title_full_unstemmed |
Geographic and Phylogenetic Variation in Bacterial Biovolume as Revealed by Protein and Nucleic Acid Staining |
title_sort |
geographic and phylogenetic variation in bacterial biovolume as revealed by protein and nucleic acid staining |
publisher |
American Society for Microbiology |
publishDate |
2009 |
url |
http://dx.doi.org/10.1128/aem.00183-09 https://journals.asm.org/doi/pdf/10.1128/AEM.00183-09 |
genre |
Antarc* Antarctic Arctic Arctic Ocean |
genre_facet |
Antarc* Antarctic Arctic Arctic Ocean |
op_source |
Applied and Environmental Microbiology volume 75, issue 12, page 4028-4034 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00183-09 |
container_title |
Applied and Environmental Microbiology |
container_volume |
75 |
container_issue |
12 |
container_start_page |
4028 |
op_container_end_page |
4034 |
_version_ |
1781692272446799872 |