Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay
ABSTRACT Magnetotactic bacteria have the unique capacity of synthesizing intracellular single-domain magnetic particles called magnetosomes. The magnetosomes are usually organized in a chain that allows the bacteria to align and swim along geomagnetic field lines, a behavior called magnetotaxis. Two...
Published in: | Applied and Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1128/aem.00165-09 https://journals.asm.org/doi/pdf/10.1128/AEM.00165-09 |
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crasmicro:10.1128/aem.00165-09 2024-04-14T08:16:22+00:00 Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay Lefèvre, Christopher T. Song, Tao Yonnet, Jean-Paul Wu, Long-Fei 2009 http://dx.doi.org/10.1128/aem.00165-09 https://journals.asm.org/doi/pdf/10.1128/AEM.00165-09 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 75, issue 12, page 3835-3841 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2009 crasmicro https://doi.org/10.1128/aem.00165-09 2024-03-15T07:50:34Z ABSTRACT Magnetotactic bacteria have the unique capacity of synthesizing intracellular single-domain magnetic particles called magnetosomes. The magnetosomes are usually organized in a chain that allows the bacteria to align and swim along geomagnetic field lines, a behavior called magnetotaxis. Two mechanisms of magnetotaxis have been described. Axial magnetotactic cells swim in both directions along magnetic field lines. In contrast, polar magnetotactic cells swim either parallel to the geomagnetic field lines toward the North Pole (north seeking) or antiparallel toward the South Pole (south seeking). In this study, we used a magnetospectrophotometry (MSP) assay to characterize both the axial magnetotaxis of “ Magnetospirillum magneticum ” strain AMB-1 and the polar magnetotaxis of magneto-ovoid strain MO-1. Two pairs of Helmholtz coils were mounted onto the cuvette holder of a common laboratory spectrophotometer to generate two mutually perpendicular homogeneous magnetic fields parallel or perpendicular to the light beam. The application of magnetic fields allowed measurements of the change in light scattering resulting from cell alignment in a magnetic field or in absorbance due to bacteria swimming across the light beam. Our results showed that MSP is a powerful tool for the determination of bacterial magnetism and the analysis of alignment and swimming of magnetotactic bacteria in magnetic fields. Moreover, this assay allowed us to characterize south-seeking derivatives and non-magnetosome-bearing strains obtained from north-seeking MO-1 cultures. Our results suggest that oxygen is a determinant factor that controls magnetotactic behavior. Article in Journal/Newspaper North Pole South pole ASM Journals (American Society for Microbiology) North Pole South Pole Applied and Environmental Microbiology 75 12 3835 3841 |
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Open Polar |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Lefèvre, Christopher T. Song, Tao Yonnet, Jean-Paul Wu, Long-Fei Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Magnetotactic bacteria have the unique capacity of synthesizing intracellular single-domain magnetic particles called magnetosomes. The magnetosomes are usually organized in a chain that allows the bacteria to align and swim along geomagnetic field lines, a behavior called magnetotaxis. Two mechanisms of magnetotaxis have been described. Axial magnetotactic cells swim in both directions along magnetic field lines. In contrast, polar magnetotactic cells swim either parallel to the geomagnetic field lines toward the North Pole (north seeking) or antiparallel toward the South Pole (south seeking). In this study, we used a magnetospectrophotometry (MSP) assay to characterize both the axial magnetotaxis of “ Magnetospirillum magneticum ” strain AMB-1 and the polar magnetotaxis of magneto-ovoid strain MO-1. Two pairs of Helmholtz coils were mounted onto the cuvette holder of a common laboratory spectrophotometer to generate two mutually perpendicular homogeneous magnetic fields parallel or perpendicular to the light beam. The application of magnetic fields allowed measurements of the change in light scattering resulting from cell alignment in a magnetic field or in absorbance due to bacteria swimming across the light beam. Our results showed that MSP is a powerful tool for the determination of bacterial magnetism and the analysis of alignment and swimming of magnetotactic bacteria in magnetic fields. Moreover, this assay allowed us to characterize south-seeking derivatives and non-magnetosome-bearing strains obtained from north-seeking MO-1 cultures. Our results suggest that oxygen is a determinant factor that controls magnetotactic behavior. |
format |
Article in Journal/Newspaper |
author |
Lefèvre, Christopher T. Song, Tao Yonnet, Jean-Paul Wu, Long-Fei |
author_facet |
Lefèvre, Christopher T. Song, Tao Yonnet, Jean-Paul Wu, Long-Fei |
author_sort |
Lefèvre, Christopher T. |
title |
Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay |
title_short |
Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay |
title_full |
Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay |
title_fullStr |
Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay |
title_full_unstemmed |
Characterization of Bacterial Magnetotactic Behaviors by Using a Magnetospectrophotometry Assay |
title_sort |
characterization of bacterial magnetotactic behaviors by using a magnetospectrophotometry assay |
publisher |
American Society for Microbiology |
publishDate |
2009 |
url |
http://dx.doi.org/10.1128/aem.00165-09 https://journals.asm.org/doi/pdf/10.1128/AEM.00165-09 |
geographic |
North Pole South Pole |
geographic_facet |
North Pole South Pole |
genre |
North Pole South pole |
genre_facet |
North Pole South pole |
op_source |
Applied and Environmental Microbiology volume 75, issue 12, page 3835-3841 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00165-09 |
container_title |
Applied and Environmental Microbiology |
container_volume |
75 |
container_issue |
12 |
container_start_page |
3835 |
op_container_end_page |
3841 |
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1796315024526934016 |