Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula
ABSTRACT Heterotrophic bacteria are well known to be key players in the turnover of dissolved organic material (DOM) in the oceans, but the relationship between DOM uptake and bacterial clades is still not well understood. Here we explore the turnover and single-cell use of glucose, an amino acid mi...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2014
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1128/aem.00121-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00121-14 |
id |
crasmicro:10.1128/aem.00121-14 |
---|---|
record_format |
openpolar |
spelling |
crasmicro:10.1128/aem.00121-14 2024-04-07T07:47:29+00:00 Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. Kostka, J. E. 2014 http://dx.doi.org/10.1128/aem.00121-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00121-14 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 80, issue 11, page 3362-3368 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2014 crasmicro https://doi.org/10.1128/aem.00121-14 2024-03-08T00:23:51Z ABSTRACT Heterotrophic bacteria are well known to be key players in the turnover of dissolved organic material (DOM) in the oceans, but the relationship between DOM uptake and bacterial clades is still not well understood. Here we explore the turnover and single-cell use of glucose, an amino acid mixture, N -acetylglucosamine (NAG), and protein by gammaproteobacterial clades in coastal waters of the West Antarctic Peninsula in summer and fall. More than 60% of the cells within two closely related gammaproteobacterial clades, Ant4D3 and Arctic96B-16, were active in using the amino acid mixture, protein, and NAG. In contrast, an average of only 7% of all SAR86 cells used amino acids and protein even in summer when DOM use was high. In addition to DOM uptake within a group, we explored the contribution of the three gammaproteobacterial groups to total community uptake of a compound. SAR86 contributed 5- to 10-fold less than the other gammaproteobacterial subgroups to the uptake of all compounds. We found that the overall contribution of the Ant4D3 clade to DOM uptake was highest, whereas the SAR86 clade contributed the least to DOM turnover in West Antarctic Peninsula waters. Our results suggest that the low growth activity of a bacterial clade leads to low abundance, fewer active cells and a low contribution to the turnover of DOM components. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula ASM Journals (American Society for Microbiology) Antarctic Antarctic Peninsula Applied and Environmental Microbiology 80 11 3362 3368 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Heterotrophic bacteria are well known to be key players in the turnover of dissolved organic material (DOM) in the oceans, but the relationship between DOM uptake and bacterial clades is still not well understood. Here we explore the turnover and single-cell use of glucose, an amino acid mixture, N -acetylglucosamine (NAG), and protein by gammaproteobacterial clades in coastal waters of the West Antarctic Peninsula in summer and fall. More than 60% of the cells within two closely related gammaproteobacterial clades, Ant4D3 and Arctic96B-16, were active in using the amino acid mixture, protein, and NAG. In contrast, an average of only 7% of all SAR86 cells used amino acids and protein even in summer when DOM use was high. In addition to DOM uptake within a group, we explored the contribution of the three gammaproteobacterial groups to total community uptake of a compound. SAR86 contributed 5- to 10-fold less than the other gammaproteobacterial subgroups to the uptake of all compounds. We found that the overall contribution of the Ant4D3 clade to DOM uptake was highest, whereas the SAR86 clade contributed the least to DOM turnover in West Antarctic Peninsula waters. Our results suggest that the low growth activity of a bacterial clade leads to low abundance, fewer active cells and a low contribution to the turnover of DOM components. |
author2 |
Kostka, J. E. |
format |
Article in Journal/Newspaper |
author |
Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. |
author_facet |
Nikrad, Mrinalini P. Cottrell, Matthew T. Kirchman, David L. |
author_sort |
Nikrad, Mrinalini P. |
title |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_short |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_full |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_fullStr |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_full_unstemmed |
Uptake of Dissolved Organic Carbon by Gammaproteobacterial Subgroups in Coastal Waters of the West Antarctic Peninsula |
title_sort |
uptake of dissolved organic carbon by gammaproteobacterial subgroups in coastal waters of the west antarctic peninsula |
publisher |
American Society for Microbiology |
publishDate |
2014 |
url |
http://dx.doi.org/10.1128/aem.00121-14 https://journals.asm.org/doi/pdf/10.1128/AEM.00121-14 |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
Applied and Environmental Microbiology volume 80, issue 11, page 3362-3368 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00121-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
11 |
container_start_page |
3362 |
op_container_end_page |
3368 |
_version_ |
1795674621359423488 |