Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea
ABSTRACT Submarine mud volcanoes are formed by expulsions of mud, fluids, and gases from deeply buried subsurface sources. They are highly reduced benthic habitats and often associated with intensive methane seepage. In this study, the microbial diversity and community structure in methane-rich sedi...
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crasmicro:10.1128/aem.00016-07 2024-10-20T14:07:53+00:00 Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea Lösekann, Tina Knittel, Katrin Nadalig, Thierry Fuchs, Bernhard Niemann, Helge Boetius, Antje Amann, Rudolf 2007 http://dx.doi.org/10.1128/aem.00016-07 https://journals.asm.org/doi/pdf/10.1128/AEM.00016-07 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 73, issue 10, page 3348-3362 ISSN 0099-2240 1098-5336 journal-article 2007 crasmicro https://doi.org/10.1128/aem.00016-07 2024-09-23T04:07:37Z ABSTRACT Submarine mud volcanoes are formed by expulsions of mud, fluids, and gases from deeply buried subsurface sources. They are highly reduced benthic habitats and often associated with intensive methane seepage. In this study, the microbial diversity and community structure in methane-rich sediments of the Haakon Mosby Mud Volcano (HMMV) were investigated by comparative sequence analysis of 16S rRNA genes and fluorescence in situ hybridization. In the active volcano center, which has a diameter of about 500 m, the main methane-consuming process was bacterial aerobic oxidation. In this zone, aerobic methanotrophs belonging to three bacterial clades closely affiliated with Methylobacter and Methylophaga species accounted for 56% ± 8% of total cells. In sediments below Beggiatoa mats encircling the center of the HMMV, methanotrophic archaea of the ANME-3 clade dominated the zone of anaerobic methane oxidation. ANME-3 archaea form cell aggregates mostly associated with sulfate-reducing bacteria of the Desulfobulbus (DBB) branch. These ANME-3/DBB aggregates were highly abundant and accounted for up to 94% ± 2% of total microbial biomass at 2 to 3 cm below the surface. ANME-3/DBB aggregates could be further enriched by flow cytometry to identify their phylogenetic relationships. At the outer rim of the mud volcano, the seafloor was colonized by tubeworms ( Siboglinidae , formerly known as Pogonophora ). Here, both aerobic and anaerobic methane oxidizers were found, however, in lower abundances. The level of microbial diversity at this site was higher than that at the central and Beggiatoa species-covered part of the HMMV. Analysis of methyl-coenzyme M-reductase alpha subunit ( mcrA ) genes showed a strong dominance of a novel lineage, mcrA group f, which could be assigned to ANME-3 archaea. Our results further support the hypothesis of Niemann et al. (54), that high methane availability and different fluid flow regimens at the HMMV provide distinct niches for aerobic and anaerobic methanotrophs. Article in Journal/Newspaper Barents Sea ASM Journals (American Society for Microbiology) Barents Sea Applied and Environmental Microbiology 73 10 3348 3362 |
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ASM Journals (American Society for Microbiology) |
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crasmicro |
language |
English |
description |
ABSTRACT Submarine mud volcanoes are formed by expulsions of mud, fluids, and gases from deeply buried subsurface sources. They are highly reduced benthic habitats and often associated with intensive methane seepage. In this study, the microbial diversity and community structure in methane-rich sediments of the Haakon Mosby Mud Volcano (HMMV) were investigated by comparative sequence analysis of 16S rRNA genes and fluorescence in situ hybridization. In the active volcano center, which has a diameter of about 500 m, the main methane-consuming process was bacterial aerobic oxidation. In this zone, aerobic methanotrophs belonging to three bacterial clades closely affiliated with Methylobacter and Methylophaga species accounted for 56% ± 8% of total cells. In sediments below Beggiatoa mats encircling the center of the HMMV, methanotrophic archaea of the ANME-3 clade dominated the zone of anaerobic methane oxidation. ANME-3 archaea form cell aggregates mostly associated with sulfate-reducing bacteria of the Desulfobulbus (DBB) branch. These ANME-3/DBB aggregates were highly abundant and accounted for up to 94% ± 2% of total microbial biomass at 2 to 3 cm below the surface. ANME-3/DBB aggregates could be further enriched by flow cytometry to identify their phylogenetic relationships. At the outer rim of the mud volcano, the seafloor was colonized by tubeworms ( Siboglinidae , formerly known as Pogonophora ). Here, both aerobic and anaerobic methane oxidizers were found, however, in lower abundances. The level of microbial diversity at this site was higher than that at the central and Beggiatoa species-covered part of the HMMV. Analysis of methyl-coenzyme M-reductase alpha subunit ( mcrA ) genes showed a strong dominance of a novel lineage, mcrA group f, which could be assigned to ANME-3 archaea. Our results further support the hypothesis of Niemann et al. (54), that high methane availability and different fluid flow regimens at the HMMV provide distinct niches for aerobic and anaerobic methanotrophs. |
format |
Article in Journal/Newspaper |
author |
Lösekann, Tina Knittel, Katrin Nadalig, Thierry Fuchs, Bernhard Niemann, Helge Boetius, Antje Amann, Rudolf |
spellingShingle |
Lösekann, Tina Knittel, Katrin Nadalig, Thierry Fuchs, Bernhard Niemann, Helge Boetius, Antje Amann, Rudolf Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea |
author_facet |
Lösekann, Tina Knittel, Katrin Nadalig, Thierry Fuchs, Bernhard Niemann, Helge Boetius, Antje Amann, Rudolf |
author_sort |
Lösekann, Tina |
title |
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea |
title_short |
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea |
title_full |
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea |
title_fullStr |
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea |
title_full_unstemmed |
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea |
title_sort |
diversity and abundance of aerobic and anaerobic methane oxidizers at the haakon mosby mud volcano, barents sea |
publisher |
American Society for Microbiology |
publishDate |
2007 |
url |
http://dx.doi.org/10.1128/aem.00016-07 https://journals.asm.org/doi/pdf/10.1128/AEM.00016-07 |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Applied and Environmental Microbiology volume 73, issue 10, page 3348-3362 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.00016-07 |
container_title |
Applied and Environmental Microbiology |
container_volume |
73 |
container_issue |
10 |
container_start_page |
3348 |
op_container_end_page |
3362 |
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1813446849205370880 |