Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim
The folic acid analog trimethoprim has been in clinical use for more than 10 years. The use of it in Sweden has doubled in the last 6 to 7 years, and from the distribution statistics it can be calculated that during 1 year 4 to 5% of the population in Sweden are given this drug. The bacterial resist...
Published in: | Antimicrobial Agents and Chemotherapy |
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American Society for Microbiology
1985
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Online Access: | http://dx.doi.org/10.1128/aac.27.6.933 https://journals.asm.org/doi/pdf/10.1128/AAC.27.6.933 |
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crasmicro:10.1128/aac.27.6.933 2023-05-15T17:44:48+02:00 Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim Steen, R Sköld, O 1985 http://dx.doi.org/10.1128/aac.27.6.933 https://journals.asm.org/doi/pdf/10.1128/AAC.27.6.933 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Antimicrobial Agents and Chemotherapy volume 27, issue 6, page 933-937 ISSN 0066-4804 1098-6596 Infectious Diseases Pharmacology (medical) Pharmacology journal-article 1985 crasmicro https://doi.org/10.1128/aac.27.6.933 2022-10-30T09:13:53Z The folic acid analog trimethoprim has been in clinical use for more than 10 years. The use of it in Sweden has doubled in the last 6 to 7 years, and from the distribution statistics it can be calculated that during 1 year 4 to 5% of the population in Sweden are given this drug. The bacterial resistance mechanisms to be found in response to such a selection pressure were investigated in a relatively isolated population in northern Sweden (the county of Jämtland), in which one centrally located bacteriological laboratory serves the area. Trimethoprim-resistant strains were collected during an 8-month period from consecutive specimens of bacteria from the urinary tracts of patients. Among the highly resistant strains of enteric bacteria, trimethoprim resistance mediated by transposon-borne dihydrofolate reductase of type I was found to dominate. The corresponding Tn7-like transposon was found to be localized both on the chromosome of isolated Escherichia coli strains and also on a 50-kilobase IncI transferable plasmid which was found in several different serotypes of E. coli. In two enterobacterial strains, resistance to more than 10(3) micrograms of trimethoprim per ml was furthermore found to be caused by a ca. 80-fold increase in the formation of chromosomal dihydrofolate reductase. Article in Journal/Newspaper Northern Sweden ASM Journals (American Society for Microbiology - via Crossref) Antimicrobial Agents and Chemotherapy 27 6 933 937 |
institution |
Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Infectious Diseases Pharmacology (medical) Pharmacology |
spellingShingle |
Infectious Diseases Pharmacology (medical) Pharmacology Steen, R Sköld, O Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim |
topic_facet |
Infectious Diseases Pharmacology (medical) Pharmacology |
description |
The folic acid analog trimethoprim has been in clinical use for more than 10 years. The use of it in Sweden has doubled in the last 6 to 7 years, and from the distribution statistics it can be calculated that during 1 year 4 to 5% of the population in Sweden are given this drug. The bacterial resistance mechanisms to be found in response to such a selection pressure were investigated in a relatively isolated population in northern Sweden (the county of Jämtland), in which one centrally located bacteriological laboratory serves the area. Trimethoprim-resistant strains were collected during an 8-month period from consecutive specimens of bacteria from the urinary tracts of patients. Among the highly resistant strains of enteric bacteria, trimethoprim resistance mediated by transposon-borne dihydrofolate reductase of type I was found to dominate. The corresponding Tn7-like transposon was found to be localized both on the chromosome of isolated Escherichia coli strains and also on a 50-kilobase IncI transferable plasmid which was found in several different serotypes of E. coli. In two enterobacterial strains, resistance to more than 10(3) micrograms of trimethoprim per ml was furthermore found to be caused by a ca. 80-fold increase in the formation of chromosomal dihydrofolate reductase. |
format |
Article in Journal/Newspaper |
author |
Steen, R Sköld, O |
author_facet |
Steen, R Sköld, O |
author_sort |
Steen, R |
title |
Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim |
title_short |
Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim |
title_full |
Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim |
title_fullStr |
Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim |
title_full_unstemmed |
Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim |
title_sort |
plasmid-borne or chromosomally mediated resistance by tn7 is the most common response to ubiquitous use of trimethoprim |
publisher |
American Society for Microbiology |
publishDate |
1985 |
url |
http://dx.doi.org/10.1128/aac.27.6.933 https://journals.asm.org/doi/pdf/10.1128/AAC.27.6.933 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Antimicrobial Agents and Chemotherapy volume 27, issue 6, page 933-937 ISSN 0066-4804 1098-6596 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aac.27.6.933 |
container_title |
Antimicrobial Agents and Chemotherapy |
container_volume |
27 |
container_issue |
6 |
container_start_page |
933 |
op_container_end_page |
937 |
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1766147090671468544 |