Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates
ABSTRACT Kingella kingae is a human pathogen that causes pediatric osteoarticular infections and infective endocarditis in children and adults. The bacterium is usually susceptible to β-lactam antibiotics, although β-lactam resistance has been reported in rare isolates. This study was conducted to i...
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crasmicro:10.1128/aac.00318-13 2023-11-05T03:42:58+01:00 Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates Banerjee, Anushree Kaplan, Jeffrey B. Soherwardy, Amenah Nudell, Yoav Mackenzie, Grace A. Johnson, Shannon Balashova, Nataliya V. 2013 http://dx.doi.org/10.1128/aac.00318-13 https://journals.asm.org/doi/pdf/10.1128/AAC.00318-13 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Antimicrobial Agents and Chemotherapy volume 57, issue 9, page 4300-4306 ISSN 0066-4804 1098-6596 Infectious Diseases Pharmacology (medical) Pharmacology journal-article 2013 crasmicro https://doi.org/10.1128/aac.00318-13 2023-10-09T16:13:16Z ABSTRACT Kingella kingae is a human pathogen that causes pediatric osteoarticular infections and infective endocarditis in children and adults. The bacterium is usually susceptible to β-lactam antibiotics, although β-lactam resistance has been reported in rare isolates. This study was conducted to identify β-lactam-resistant strains and to characterize the resistance mechanism. Screening of a set of 90 K. kingae clinical isolates obtained from different geographic locations revealed high-level resistance to penicillins among 25% of the strains isolated from Minnesota and Iceland. These strains produced TEM-1 β-lactamase and were shown to contain additional ≥50-kb plasmids. Ion Torrent sequencing of extrachromosomal DNA from a β-lactamase-producing strain confirmed the plasmid location of the bla TEM gene. An identical plasmid pattern was demonstrated by multiplex PCR in all β-lactamase producers. The porin gene's fragments were analyzed to investigate the relatedness of bacterial strains. Phylogenetic analysis revealed 27 single-nucleotide polymorphisms (SNPs) in the por gene fragment, resulting in two major clusters with 11 allele types forming bacterial-strain subclusters. β-Lactamase producers were grouped together based on por genotyping. Our results suggest that the β-lactamase-producing strains likely originate from a single plasmid-bearing K. kingae isolate that traveled from Europe to the United States, or vice versa. This study highlights the prevalence of penicillin resistance among K. kingae strains in some regions and emphasizes the importance of surveillance for antibiotic resistance of the pathogen. Article in Journal/Newspaper Iceland ASM Journals (American Society for Microbiology - via Crossref) Antimicrobial Agents and Chemotherapy 57 9 4300 4306 |
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Infectious Diseases Pharmacology (medical) Pharmacology |
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Infectious Diseases Pharmacology (medical) Pharmacology Banerjee, Anushree Kaplan, Jeffrey B. Soherwardy, Amenah Nudell, Yoav Mackenzie, Grace A. Johnson, Shannon Balashova, Nataliya V. Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates |
topic_facet |
Infectious Diseases Pharmacology (medical) Pharmacology |
description |
ABSTRACT Kingella kingae is a human pathogen that causes pediatric osteoarticular infections and infective endocarditis in children and adults. The bacterium is usually susceptible to β-lactam antibiotics, although β-lactam resistance has been reported in rare isolates. This study was conducted to identify β-lactam-resistant strains and to characterize the resistance mechanism. Screening of a set of 90 K. kingae clinical isolates obtained from different geographic locations revealed high-level resistance to penicillins among 25% of the strains isolated from Minnesota and Iceland. These strains produced TEM-1 β-lactamase and were shown to contain additional ≥50-kb plasmids. Ion Torrent sequencing of extrachromosomal DNA from a β-lactamase-producing strain confirmed the plasmid location of the bla TEM gene. An identical plasmid pattern was demonstrated by multiplex PCR in all β-lactamase producers. The porin gene's fragments were analyzed to investigate the relatedness of bacterial strains. Phylogenetic analysis revealed 27 single-nucleotide polymorphisms (SNPs) in the por gene fragment, resulting in two major clusters with 11 allele types forming bacterial-strain subclusters. β-Lactamase producers were grouped together based on por genotyping. Our results suggest that the β-lactamase-producing strains likely originate from a single plasmid-bearing K. kingae isolate that traveled from Europe to the United States, or vice versa. This study highlights the prevalence of penicillin resistance among K. kingae strains in some regions and emphasizes the importance of surveillance for antibiotic resistance of the pathogen. |
format |
Article in Journal/Newspaper |
author |
Banerjee, Anushree Kaplan, Jeffrey B. Soherwardy, Amenah Nudell, Yoav Mackenzie, Grace A. Johnson, Shannon Balashova, Nataliya V. |
author_facet |
Banerjee, Anushree Kaplan, Jeffrey B. Soherwardy, Amenah Nudell, Yoav Mackenzie, Grace A. Johnson, Shannon Balashova, Nataliya V. |
author_sort |
Banerjee, Anushree |
title |
Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates |
title_short |
Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates |
title_full |
Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates |
title_fullStr |
Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates |
title_full_unstemmed |
Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates |
title_sort |
characterization of tem-1 β-lactamase-producing kingella kingae clinical isolates |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://dx.doi.org/10.1128/aac.00318-13 https://journals.asm.org/doi/pdf/10.1128/AAC.00318-13 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Antimicrobial Agents and Chemotherapy volume 57, issue 9, page 4300-4306 ISSN 0066-4804 1098-6596 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aac.00318-13 |
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Antimicrobial Agents and Chemotherapy |
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57 |
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9 |
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