Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells
With the ability of self-renewal and multipotenntial differentiation, muscle satellite cells (MSCs) are recognized as a population of tissue-specific progenitors which play an important role in the growth, repair and regeneration of muscles. Satellite cells have been isolated for a very long time, b...
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crascipubl:10.1166/jbt.2019.2030 2023-05-15T15:50:29+02:00 Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells Lu, Tengfei Wang, Xishuai Zheng, Yanjie Wen, Hebao Ji, Hongda Guan, Weijun 2019 http://dx.doi.org/10.1166/jbt.2019.2030 https://www.ingentaconnect.com/content/asp/jbte/2019/00000009/00000005/art00002 en eng American Scientific Publishers Journal of Biomaterials and Tissue Engineering volume 9, issue 5, page 573-582 ISSN 2157-9083 Biomedical Engineering Medicine (miscellaneous) Bioengineering Biotechnology journal-article 2019 crascipubl https://doi.org/10.1166/jbt.2019.2030 2022-08-09T17:27:49Z With the ability of self-renewal and multipotenntial differentiation, muscle satellite cells (MSCs) are recognized as a population of tissue-specific progenitors which play an important role in the growth, repair and regeneration of muscles. Satellite cells have been isolated for a very long time, but the techniques of isolation are still to be improved. In this study, MSCs were obtained from aborted Tibetan mastiff fetuses (about 60 days) under sterile conditions. Primary MSCs were sub-cultured to passage 25 in vitro . The gene of C-met, MyoD1, Desmin and Pax7 were detected by RT-polymerase chain reaction and immunofluorescence assays. The result showed that they were positive in MSCs of Tibetan mastiff. The growth of different passages cells typically appeared in S curve. Furthermore, myogenic, osteogenic and adipogenic differenciation of MSCs were successfully induced. The results denoted that the MSCs obtained from Tibetan Mastiffs exhibited the characteristics of multipotent stem cells. Therefore, this work provided a theoretical and technical basis for Tibetan mastiff, even its genetic resources preservation and laid a solid foundation for studying the mechanism of skeletal muscle growth and development in future. Article in Journal/Newspaper Canis lupus American Scientific Publishers (via Crossref) Journal of Biomaterials and Tissue Engineering 9 5 573 582 |
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American Scientific Publishers (via Crossref) |
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English |
topic |
Biomedical Engineering Medicine (miscellaneous) Bioengineering Biotechnology |
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Biomedical Engineering Medicine (miscellaneous) Bioengineering Biotechnology Lu, Tengfei Wang, Xishuai Zheng, Yanjie Wen, Hebao Ji, Hongda Guan, Weijun Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells |
topic_facet |
Biomedical Engineering Medicine (miscellaneous) Bioengineering Biotechnology |
description |
With the ability of self-renewal and multipotenntial differentiation, muscle satellite cells (MSCs) are recognized as a population of tissue-specific progenitors which play an important role in the growth, repair and regeneration of muscles. Satellite cells have been isolated for a very long time, but the techniques of isolation are still to be improved. In this study, MSCs were obtained from aborted Tibetan mastiff fetuses (about 60 days) under sterile conditions. Primary MSCs were sub-cultured to passage 25 in vitro . The gene of C-met, MyoD1, Desmin and Pax7 were detected by RT-polymerase chain reaction and immunofluorescence assays. The result showed that they were positive in MSCs of Tibetan mastiff. The growth of different passages cells typically appeared in S curve. Furthermore, myogenic, osteogenic and adipogenic differenciation of MSCs were successfully induced. The results denoted that the MSCs obtained from Tibetan Mastiffs exhibited the characteristics of multipotent stem cells. Therefore, this work provided a theoretical and technical basis for Tibetan mastiff, even its genetic resources preservation and laid a solid foundation for studying the mechanism of skeletal muscle growth and development in future. |
format |
Article in Journal/Newspaper |
author |
Lu, Tengfei Wang, Xishuai Zheng, Yanjie Wen, Hebao Ji, Hongda Guan, Weijun |
author_facet |
Lu, Tengfei Wang, Xishuai Zheng, Yanjie Wen, Hebao Ji, Hongda Guan, Weijun |
author_sort |
Lu, Tengfei |
title |
Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells |
title_short |
Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells |
title_full |
Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells |
title_fullStr |
Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells |
title_full_unstemmed |
Isolation, Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells |
title_sort |
isolation, culture and biological characteristics of tibetan mastiff (canis lupus familiaris) muscle satellite cells |
publisher |
American Scientific Publishers |
publishDate |
2019 |
url |
http://dx.doi.org/10.1166/jbt.2019.2030 https://www.ingentaconnect.com/content/asp/jbte/2019/00000009/00000005/art00002 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Journal of Biomaterials and Tissue Engineering volume 9, issue 5, page 573-582 ISSN 2157-9083 |
op_doi |
https://doi.org/10.1166/jbt.2019.2030 |
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Journal of Biomaterials and Tissue Engineering |
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9 |
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5 |
container_start_page |
573 |
op_container_end_page |
582 |
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1766385431036821504 |