Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography
Autoradiography with tritiated thymidine was used to study microbial growth directly in nature. The epiphyte Leucothrix mucor was used since it is a large filamentous bacterium with a characteristic morphology making it recognizable in natural collections. The technique was developed initially with...
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American Association for the Advancement of Science (AAAS)
1967
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craaas:10.1126/science.155.3758.81 2024-06-09T07:47:10+00:00 Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography Brock, Thomas D. 1967 http://dx.doi.org/10.1126/science.155.3758.81 https://www.science.org/doi/pdf/10.1126/science.155.3758.81 en eng American Association for the Advancement of Science (AAAS) Science volume 155, issue 3758, page 81-83 ISSN 0036-8075 1095-9203 journal-article 1967 craaas https://doi.org/10.1126/science.155.3758.81 2024-05-16T12:55:02Z Autoradiography with tritiated thymidine was used to study microbial growth directly in nature. The epiphyte Leucothrix mucor was used since it is a large filamentous bacterium with a characteristic morphology making it recognizable in natural collections. The technique was developed initially with pure cultures. The relation between growth rate and the rate of accumulation of radioactive cells permitted derivation of a constant for use in calculating growth rate in natural material and in two-membered cultures of L. mucor growing epiphytically on pure cultures of marine algae. The growth rate (generation time) in two-membered cultures with the red alga Antithamnion sarniense was 94 minutes under the conditions used. In nature the growth rate of a sample from Iceland was 685 minutes; that of a sample from Long Island Sound was 660 minutes. There was no evidence of preferential growth in the basal portion of bacterial filaments nearest the algal surface. However, filamentous growth in nature, but not in pure or two-membered culture, was nonrandom, growth being clustered in some regions. Article in Journal/Newspaper Iceland AAAS Resource Center (American Association for the Advancement of Science) Long Island Long Island Sound ENVELOPE(-79.366,-79.366,54.800,54.800) Science 155 3758 81 83 |
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Open Polar |
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AAAS Resource Center (American Association for the Advancement of Science) |
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craaas |
language |
English |
description |
Autoradiography with tritiated thymidine was used to study microbial growth directly in nature. The epiphyte Leucothrix mucor was used since it is a large filamentous bacterium with a characteristic morphology making it recognizable in natural collections. The technique was developed initially with pure cultures. The relation between growth rate and the rate of accumulation of radioactive cells permitted derivation of a constant for use in calculating growth rate in natural material and in two-membered cultures of L. mucor growing epiphytically on pure cultures of marine algae. The growth rate (generation time) in two-membered cultures with the red alga Antithamnion sarniense was 94 minutes under the conditions used. In nature the growth rate of a sample from Iceland was 685 minutes; that of a sample from Long Island Sound was 660 minutes. There was no evidence of preferential growth in the basal portion of bacterial filaments nearest the algal surface. However, filamentous growth in nature, but not in pure or two-membered culture, was nonrandom, growth being clustered in some regions. |
format |
Article in Journal/Newspaper |
author |
Brock, Thomas D. |
spellingShingle |
Brock, Thomas D. Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography |
author_facet |
Brock, Thomas D. |
author_sort |
Brock, Thomas D. |
title |
Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography |
title_short |
Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography |
title_full |
Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography |
title_fullStr |
Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography |
title_full_unstemmed |
Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography |
title_sort |
bacterial growth rate in the sea: direct analysis by thymidine autoradiography |
publisher |
American Association for the Advancement of Science (AAAS) |
publishDate |
1967 |
url |
http://dx.doi.org/10.1126/science.155.3758.81 https://www.science.org/doi/pdf/10.1126/science.155.3758.81 |
long_lat |
ENVELOPE(-79.366,-79.366,54.800,54.800) |
geographic |
Long Island Long Island Sound |
geographic_facet |
Long Island Long Island Sound |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Science volume 155, issue 3758, page 81-83 ISSN 0036-8075 1095-9203 |
op_doi |
https://doi.org/10.1126/science.155.3758.81 |
container_title |
Science |
container_volume |
155 |
container_issue |
3758 |
container_start_page |
81 |
op_container_end_page |
83 |
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1801378097146101760 |