Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series
UCYN-A is a globally important nitrogen-fixing symbiotic microbe often found in colder regions and coastal areas where nitrogen fixation has been overlooked. We present a 3-year coastal Northwest Atlantic time series of UCYN-A by integrating oceanographic data with weekly nifH and16 S rRNA gene sequ...
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craaas:10.1126/sciadv.adh9768 2024-06-09T07:48:38+00:00 Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series Robicheau, Brent M. Tolman, Jennifer Desai, Dhwani LaRoche, Julie 2023 http://dx.doi.org/10.1126/sciadv.adh9768 https://www.science.org/doi/pdf/10.1126/sciadv.adh9768 en eng American Association for the Advancement of Science (AAAS) Science Advances volume 9, issue 39 ISSN 2375-2548 journal-article 2023 craaas https://doi.org/10.1126/sciadv.adh9768 2024-05-16T12:55:18Z UCYN-A is a globally important nitrogen-fixing symbiotic microbe often found in colder regions and coastal areas where nitrogen fixation has been overlooked. We present a 3-year coastal Northwest Atlantic time series of UCYN-A by integrating oceanographic data with weekly nifH and16 S rRNA gene sequencing and quantitative PCR assays for UCYN-A ecotypes. High UCYN-A relative abundances dominated by A1 to A4 ecotypes reoccurred annually in the coastal Northwest Atlantic. Although UCYN-A was detected every summer/fall, the ability to observe separate ecotypes may be highly dependent on sampling time given intense interannual and weekly variability of ecotype-specific occurrences. Additionally, much of UCYN-A’s rarer diversity was populated by short-lived neutral mutational variants, therefore providing insight into UCYN-A’s microevolutionary patterns. For instance, rare ASVs exhibited community composition restructuring annually, while also sharing a common connection to a dominant ASV within each ecotype. Our study provides additional perspectives for interpreting UCYN-A intraspecific diversity and underscores the need for high-resolution datasets when deciphering spatiotemporal ecologies within UCYN-A. Article in Journal/Newspaper Northwest Atlantic AAAS Resource Center (American Association for the Advancement of Science) Science Advances 9 39 |
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Open Polar |
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AAAS Resource Center (American Association for the Advancement of Science) |
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craaas |
language |
English |
description |
UCYN-A is a globally important nitrogen-fixing symbiotic microbe often found in colder regions and coastal areas where nitrogen fixation has been overlooked. We present a 3-year coastal Northwest Atlantic time series of UCYN-A by integrating oceanographic data with weekly nifH and16 S rRNA gene sequencing and quantitative PCR assays for UCYN-A ecotypes. High UCYN-A relative abundances dominated by A1 to A4 ecotypes reoccurred annually in the coastal Northwest Atlantic. Although UCYN-A was detected every summer/fall, the ability to observe separate ecotypes may be highly dependent on sampling time given intense interannual and weekly variability of ecotype-specific occurrences. Additionally, much of UCYN-A’s rarer diversity was populated by short-lived neutral mutational variants, therefore providing insight into UCYN-A’s microevolutionary patterns. For instance, rare ASVs exhibited community composition restructuring annually, while also sharing a common connection to a dominant ASV within each ecotype. Our study provides additional perspectives for interpreting UCYN-A intraspecific diversity and underscores the need for high-resolution datasets when deciphering spatiotemporal ecologies within UCYN-A. |
format |
Article in Journal/Newspaper |
author |
Robicheau, Brent M. Tolman, Jennifer Desai, Dhwani LaRoche, Julie |
spellingShingle |
Robicheau, Brent M. Tolman, Jennifer Desai, Dhwani LaRoche, Julie Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series |
author_facet |
Robicheau, Brent M. Tolman, Jennifer Desai, Dhwani LaRoche, Julie |
author_sort |
Robicheau, Brent M. |
title |
Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series |
title_short |
Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series |
title_full |
Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series |
title_fullStr |
Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series |
title_full_unstemmed |
Microevolutionary patterns in ecotypes of the symbiotic cyanobacterium UCYN-A revealed from a Northwest Atlantic coastal time series |
title_sort |
microevolutionary patterns in ecotypes of the symbiotic cyanobacterium ucyn-a revealed from a northwest atlantic coastal time series |
publisher |
American Association for the Advancement of Science (AAAS) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1126/sciadv.adh9768 https://www.science.org/doi/pdf/10.1126/sciadv.adh9768 |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
Science Advances volume 9, issue 39 ISSN 2375-2548 |
op_doi |
https://doi.org/10.1126/sciadv.adh9768 |
container_title |
Science Advances |
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9 |
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39 |
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1801380436175224832 |